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This study explores the interactions between HA-UNG2, Vpr, and tubulin in the context of Flag-DCAF1 presence and absence. Using various constructs including HA-UNG2, HA-Vpr, and no Vpr variations, we delve into the cellular implications of these interactions. Results indicate significant changes in tubulin stabilization and signaling pathways. This research provides insights into the molecular mechanisms by which viral proteins like Vpr influence host cell machinery, potentially impacting viral pathogenesis.
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Additional file 4: Figure S4 HA-UNG2 Vpr Tubulin HA-UNG2 - + + + + + + + + Flag-DCAF1 - - + + + + + + + NoVpr HA-Vpr SIVagm HA-Vpr