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ISOLATION

Nov 13, 2014

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MATERIAL. Primary tumor. Cell line. Mouse xenograft. CD44 + /CD24 -/low /lin - ALDEFLUOR assay Side population. CD133? CD61? CD49f/CD29?. R1. R1. R2. R2. ISOLATION. FACS analysis. in vitro assay. in vivo assay. PROPERTIES. Tumorigenicity assay

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ISOLATION

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MATERIAL Primary tumor Cell line Mouse xenograft • CD44+/CD24-/low/lin- • ALDEFLUOR assay • Side population • CD133? • CD61? • CD49f/CD29? R1 R1 R2 R2 ISOLATION FACS analysis in vitro assay in vivo assay PROPERTIES • Tumorigenicity assay • Recapitulation of the initial phenotype • Serial passages (self-renewal) • Tumorsphere formation • Serial passages (self-renewal)

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