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Evolution and Expression Profiling of PPHLN1 Gene in Primates Involving HERV-M Elements

This research examines the evolutionary dynamics and expression profiles of the PPHLN1 gene across primates, focusing on the role of HERV-M retroelements. By enhancing the LTR structures beyond existing Repbase sequences, a comprehensive consensus sequence was established. We analyzed insertion timings and their contributions to PPHLN1 diversification. Expression profiling across various tissues like brain, prostate, and liver reveals alternative transcripts related to HERV-M, emphasizing significant evolutionary adaptations spanning millions of years.

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Evolution and Expression Profiling of PPHLN1 Gene in Primates Involving HERV-M Elements

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Presentation Transcript

  1. Produced by Huh Jae-won

  2. 구조: 특이점은 가상의 코팅지역을 만들었다.

  3. 분포와 삽입된 구조

  4. LTR을 기존의 Repbase에 있는것보다 더 보강해서 컨센선스 서열을 만들었다.

  5. 진화적 분석과 삽입시기

  6. Retroelement(HERV-M) contribute to the diversification of PPHLN1 gene during the Primate evolution

  7. Expression Profiling of HERV-M related alternative transcript Brain Prostate Testis Kidney Heart Liver Lung Placenta Ske-M Spleen Thymus Uterus Human Chimp Go Or Gi Rhesus Hu AT Hu Bo Rhe AT Rhe Bo Cord B Rhe Hi 1 2

  8. Human Chimpanzee Gorilla Orangutan Gibbon Old World monkeys New World monkeys Prosimians Million years 60 50 40 30 20 10 Evolution of PPHLN1 related HERV-M Divergence : 10%(±1.5%)

  9. HERVM LTR pol gag LTR IN RT RnaseH 5’ LTR 3’ LTR 1 458 620 1338 1461 2215 2644 3050 3818 4038 1 458 PBS 3928 3967 4038 1 458 1 77 3402 3626 Splicing of HERV-M

  10. Aligment of PPHLN1 Transcripts

  11. The Structure of PPHLN1 Gene LTR5B MER58A HERV-M Orthologous region Human 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Transcript variant 1 Transcript variant 2 Transcript variant 3 Transcript variant 4 Transcript variant 5 Alusq/x VS L1ME4a Transcript variant 1 AluJo Transcript variant 2 Mouse 1 2 3 4 5 6 7 8 9 10

  12. (Retroelements : Alu, Line, HERV) Hu1 Hu2 Hu3 Hu4 Type 1 transcript Mu1(Duplication) Common ancestor Hu5 Type 2 transcript Mu2 Evolution of PPHLN1 transcripts Driving Forces PPHLN1 gene

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