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Plasmid Isolation and Analysis: Restriction Digest and Gel Electrophoresis

Learn the process of isolating plasmid DNA, performing restriction digest, and running gel electrophoresis to analyze the resulting fragments. Understand enzyme cutting patterns and control reactions.

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Plasmid Isolation and Analysis: Restriction Digest and Gel Electrophoresis

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  1. MICR 300 Section 03 Experiment 14 Plasmid Isolation, Restriction Digest, and Minigel Electrophoresis 11.05.07 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8 Table 1 Table 2 1 2 3 4 5 6 7 8 8 7 6 5 4 3 2 1 Table 3 Table 4 1: plasmid + water 2: plasmid + EcoR1 (expect 2 cuts) 3: plasmid + DraI (expect multiple cuts) 4: Ladder 5: Positive control Plasmid + one enzyme (Table1,3: DraI, Table 2,4: ecoR1) 6: plasmid + water 7: plasmid + EcoR1 8: plasmid + DraI

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