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Macromolecular Crystallography

Macromolecular Crystallography. Summary of the July 18, 2007 Breakout Session. Beam line (s) - current resources. Most common beamline elements in use Optics Source: Undulators; Wigglers Monochromators: double crystal, sagital focusing, channel cut-Silicon

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Macromolecular Crystallography

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  1. Macromolecular Crystallography Summary of the July 18, 2007 Breakout Session

  2. Beam line (s) - current resources • Most common beamline elements in use • Optics • Source: Undulators; Wigglers • Monochromators: double crystal, sagital focusing, channel cut-Silicon • Mirrors: focusing, collimating; Kirk-Baez pairs • Energy range: 7 - 20 KeV • End-station • Diffractometers:Build In-house or Crystallogic (single axis, kappa); Hubbert; EMBL MicroDiffractometer* • Detectors: ADSC, MarCCD • Control: Blu(Web)ICE Science Highlights Beamlines: X6A, X29 Technique: Macromolecular Crystallography Researchers: Yongcheng Wang, Yingjiu Zhang and Ya Ha (Yale School of Medicine) Publication: “Crystal structure of a rhomboid family intramembrane protease” Nature (2006) 444, 179-180

  3. What can be done with a new source such as NSLSII • Challenges • Molecular Machines - multiple stage molecular machines • Membrane Proteins • Virus • Structural “-omics” • Drug development (vaccines, anesthetics…) • Time resolved….kinetics….dynamics • Very small less then 10 micron or most probably smaller crystals • Weakly diffracting crystals 50 ang 250 ang 750 ang

  4. My next Macromolecular Crystallography beam line(s) Beam lines Small beams, positional stability, small divergence, beam line automation. Optics Monochromator: Si 311 Mirror: KB mirrors (bimorph?) Energy range: 3(2) to 60 keV End-station Large detectors, continuous read out preferred Rotation axis with small sphere of confusion (< 1 micron) Crystal visualization/centering tools (need research) Ancillarydevices

  5. My next Macromolecular Crystallography beam line(s) Laboratories and ancillary facilities Labs Wet labs, cold rooms microscopes crystallization facilities (?) Biohazard Level (?) DI-water Mechanical, Electrical IT remote user access security e.g. through certificates large capacity data storage

  6. Quality of Life Facility meeting rooms small and medium or convertible offices for staff and visitors lunch room for users and staff Housing short stay - hotel like long stay - 1, 2, 3 Bedrooms with kitchen facilities Food vending automats - better setup then the vending machines small convenience store (18/7) Transportation campus only between BNL Stony Brook and Ronkonkoma train station (?)paid IT wireless network on campus

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