1 / 20

Structures and Structure Descriptions

Structures and Structure Descriptions. Chapter 8 Protein Bioinformatics. Protein Classes. Active – Mobility and catalysis soluble and globular in shape Passive – structural Membrane – control import and export through membrane. Folding in Globular Proteins. fold into compact units

kana
Télécharger la présentation

Structures and Structure Descriptions

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Structures and Structure Descriptions Chapter 8 Protein Bioinformatics

  2. Protein Classes • Active – Mobility and catalysis • soluble and globular in shape • Passive – structural • Membrane – control import and export through membrane

  3. Folding in Globular Proteins • fold into compact units • 100-1000 nucleotides • Stable fold has minimum energy • Native state • Energy loss occurs when bonds are formed: • H-Bonds • disulfide bridges (cysteine) • metallic bonds w/ metal ions

  4. Folding Formation of H-bonds: • Hydrophilic amino acids are soluble, hydrophobic are not • To maximize H-bonds, put hydrophilic on the surface so the whole protein is soluble • Causes the formation of the two dominant Secondary Structure Elements (SSEs): • α-helix • β-strand

  5. Structural Comparison • Fine Level (residue) • used for finding spatial similarities – active and binding sites • helpful for determining function • done by specifying coordinates, distances, or torsion angles • Coarse Level (SSE) • used for comparing on the global level • helpful for classifying proteins into classes • done by describing SSEs using line segments or as ellipsoids

  6. Process

  7. Structure Description • Architecture - position of (or Geometry) elements (atoms or residues) • Topology - order of elements along the backbone • Properties - physio-chemical properties and types of SSEs

  8. CoordinatesFrom NMR or X-Ray Crystallography Describing Fine Level Structure ATOM 1 N PRO A 2 31.242 3.064 39.284 1.00 39.90 N ATOM 2 CA PRO A 2 31.195 2.392 37.963 1.00 31.96 C ATOM 3 C PRO A 2 29.975 2.923 37.197 1.00 30.23 C ATOM 4 O PRO A 2 29.727 4.132 37.181 1.00 27.03 O ATOM 5 CB PRO A 2 31.063 0.905 38.251 1.00 36.57 C ATOM 6 CG PRO A 2 30.276 0.947 39.549 1.00 35.11 C ATOM 7 CD PRO A 2 30.829 2.121 40.343 1.00 42.06 C ATOM 8 N TYR A 3 29.189 2.020 36.613 1.00 22.83 N ATOM 9 CA TYR A 3 28.011 2.405 35.850 1.00 18.42 C ATOM 10 C TYR A 3 26.711 1.995 36.517 1.00 19.46 C ATOM 11 O TYR A 3 26.629 0.949 37.161 1.00 24.89 O ATOM 12 CB TYR A 3 28.055 1.772 34.459 1.00 17.73 C

  9. Distance Matrices Describing Fine Level Structure • Distances can be stored with 3n-6 distances instead of 4n-10 coordinates. • 2D representation of the 3D structure

  10. Torsion Angles Describing Fine Level Structure • Angles between two bonds of each atom in the backbone are approx. equal • Freedom comes in rotating around single bonds (-70,-20), (-72,60), (-70,120), (-60,170), (-65, 125), (-100, 45), (-100, -65), (-105, -66), (-100, 60)

  11. Line Segments (sticks) Describing Coarse Level Structure • Fit a line to the Catom of each residue by least squares Ellipsoids • Three inertial axes long axis corresponds to stick representation

  12. Describing Coarse Level Structure Helices • α-helix 4-turn helix, min. 4 residues 310-helix 3-turn helix, min. 3 residues π-helix 5-turn helix, min. 5 residues • Formed by H-Bonds between residues in the same helix

  13. Describing Coarse Level Structure Strands and Sheets • Formed by successive H-Bonds between residues can be far apart in sequence.

  14. Cartoons for Secondary Structure Elements (SSE) • Topology of Protein Structure (TOPS) • Triangular symbols represent beta strands • Circular symbols represent helices (alpha and 310) • The peptide chain is divided into a number of fragments each labelled with an integer (i), beginning at Ni and ending at Ci+1. • The first fragment is N1->C2 (or N->C). • Each fragment lies in only one structural domain. • Where the chain crosses between domains it leaves the first at Ci and joins the next at Ni. • Each secondary structure element has a direction (N to C) which is either "up" ( out of the plane of the diagram ) or "down" (into the plane of the diagram).

  15. Comparing Structures • Structure Representations – pg. 185-186 (11-12 of pdf) • Strings • List of unit descriptions • Set of unit descriptions • Graphs • Feature Arrays

  16. Pairwise Comparison • Finding equivalence or alignment giving highest score is NP-Complete

  17. Example • Alignment • ACSL-DRTS-IRV • A-TLREKSSLIR- • Know first 5 residues • ACSL-D • A-TLRE

  18. But not so with structures Dynamic Programming cannot be used directly for structure alignment highest score alignment of entire structures highest score alignment of first five residues

  19. Last Slide  • Explore pdb and install cn3d • http://www.rcsb.org/pdb • http://www.ncbi.nlm.nih.gov/Structure/CN3D/cn3d.shtml RCSB PDB - HUMAN GLUTATHIONE S-TRANSFERASE

More Related