Alcohol’s Effect on Short Term Memory in Drosophila Melanogaster

1. Alcohol’s Effect on Short Term Memory in Drosophila Melanogaster By Virginia Brodie

2. http://www.nt.gov.au/health/healthdev/health_promotion/bushbook/volume2/chap1/body_alcohol.gifhttp://www.nt.gov.au/health/healthdev/health_promotion/bushbook/volume2/chap1/body_alcohol.gif Need • 14 million people in the United States suffer from alcoholism • Long term drinking can lead to Wernicke-Korsakoff Syndrome

3. http://www.drgaryseeman.com/800px-Ethanol-3D-balls.png http://www.jpp.krakow.pl/journal/archive/1203_s4/gfx/rys16_2.jpg Knowledge Base-Alcohol Alcohols Effect on the Brain Ethanol Alcohol- CH3CH2OH

4. Knowledge Base- Short Term Memory Foot -Shock

5. http://www.bcm.edu/labs/davis/researchprojects.htm Olfactory Memory

6. Heberlein, Ulrike. "Genetics of Alcohol-Induced Behaviors in Drosophila." (2000). Inebriometer • Found that Drosophila have similar intoxication patterns to humans http://pubs.niaaa.nih.gov/publications/arh24-3/185-188.pdf

7. Laurie, C. C. "Genetic basis of the difference in alcohol dehydrogenase expression between Drosophila melanogaster and Drosophila simulans." (1990). • Found that flies can develop tolerance to alcohol Adh gene and fragments http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=55235&blobtype=pdf

8. Moore, Monica. "Ethanol Intoxication in Drosophila:Genetic and Pharmacological Evidence for Regulation by the cAMP Signaling Pathway." (1998). • Found that the cAMP signaling system plays a role in meditating alcohols affects Comparison between Control and chpd Drosophila Control and chpd http://www.northwestern.edu/neurobiology/faculty/allada2/gbbpapers/MooreHeberlein98.pdf

9. LaFerriere, 2008 "Genetic Dissociation of Ethanol Sensitivity and Memory Formation in Drosophila melanogaster Heat Box Test • Relation between ethanol sensitivity and learning mechanisms in Drosophila http://learnmem.cshlp.org/content/9/5/349.full.pdf • Laferriere, Holly, and Douglas J. Guarnieri. "Genetic Dissociation of Ethanol Sensitivity and Memory Formation in Drosophila melanogaster." (2008). Genetics. <http://www.genetics.org/cgi/content/full/178/4/1895/>.

10. Hypothesis Null : The ethanol exposure will have no effect on the short term memory of Drosophila melanogaster. Alternate: The Drosophila exposed to ethanol will have impaired short term memory, and the Drosophila who are not, will not (LaFerriere, 08).

11. Purpose • The purpose of this experiment is to demonstrate the effects of alcohol exposure on short term memory in drosophila.

12. Methodology Alcohol’s Effect on Short term Memory In Drosophila Canton S- flies never exposed to alcohol Canton S flies exposed to 200 proof ethanol Flies placed at top of inebriometer while being pumped 200 proof ethanol, time elapsed for flies to reach bottom is measured Flies shocked for 60-second intervals, 4 times, with 10 minutes in between, while simultaneously being pumped 3-octanol 5 hours later, the flies are lowered between 3-octanol (familiar scent) and 4- methycyclihexanol (unfamiliar), those that move away from the 3-oct are said to have formed short-term memory. Data analyzed using a performance index, to show learning, and Chi Squared, to show the significance between the expected and observed data.

13. Training and Testing Apparatus Photos taken by researcher

14. Budget

15. Do ability • Olfactory training and testing apparatus is already in the lab • Inebriometer is available in the lab • Training and testing process for memory is already known

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