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Verification of Polyclonal Antibody Specificities and Genomic Structures of OsAPX1 and OsAPX2 in IR-29

This study focuses on the verification of specificities for polyclonal antibodies targeting OsAPX1 and OsAPX2 in the IR-29 rice cultivar. Using Coomassie staining and anti-APX1 and anti-APX2 antibodies, we identified the distinct bands corresponding to the OsAPX1 (3,015 bp) and OsAPX2 (1,917 bp) genes. Additionally, we compared the genomic structures of these genes, utilizing reference sequences from GenBank. The analysis highlights nucleotide mismatches and deletions in the IR-29 cultivar, providing insights into the genetic variations specific to this strain.

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Verification of Polyclonal Antibody Specificities and Genomic Structures of OsAPX1 and OsAPX2 in IR-29

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  1. OsAPXs-IR29 M 1 1 1 2 2 2 (kD) 75 50 37 25 20 15 Fig. A1 Verification of specificities of polyclonal antibodies between OsAPX1-IR29 and OsAPX2-IR29 Coomassie anti-APX1 anti-APX2

  2. 0 1 2 3 (kb) OsAPX1 (3,015 bp) 20 bp OsAPX1-IR29 (2,995 bp) 2196 127 2827 1681 2689 2319 179 2711 (R218C) Fig. A2 Comparison of genomic structures of OsAPX1 and OsAPX2 in IR-29 cultivar. The known OsAPX1 (Accession No. NC_008396) and OsAPX2 (Accession No. NC_008400) genomic sequences available in Genbank were used as references. The number with an arrow represents the base of OsAPXs nucleotides, where the nucleotide sequences were mismatched with IR-29. A reversed triangle indicates deleted bases in IR-29 nucleotide OsAPX2 (1,917 bp) 3 bp 6 bp OsAPX2-IR29 (1,908 bp) 426 1675

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