1 / 23

Introducing Apceden ™

Introducing Apceden ™. Topics. IMMUNITY AND CANCER DENDRITIC CELL BIOLOGY Development of Dendritic Cells Why Dendritic Cells Mechanism of Action CLINICAL TRIALS APCEDEN™ Preparation Associated Logistics . IMMUNITY AND CANCER. IMMUNITY AND CANCER.

keagan
Télécharger la présentation

Introducing Apceden ™

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Introducing Apceden™

  2. Topics • IMMUNITY AND CANCER • DENDRITIC CELL BIOLOGY • Development of Dendritic Cells • Why Dendritic Cells • Mechanism of Action • CLINICAL TRIALS • APCEDEN™ • Preparation • Associated Logistics

  3. IMMUNITY AND CANCER

  4. IMMUNITY AND CANCER • Clinical trials with vaccination of ex-vivo generated dendritic cells (DCs) pulsed with tumor antigens have provided a proof-of-principle that therapeutic immunity can be elicited in cancers • However Clinical benefit has been observed in only a fraction of cases when measured by regression of tumors in stage IV cancer • The next generation of DC vaccines are expected to generate large numbers of high avidity effector CD8+ T cells to overcome regulatory T cells and the suppressive environment established by tumors • Therapeutic vaccination protocols with improved DC vaccines in combination with chemotherapy is expected to exploit immunogenic chemotherapy regimens

  5. IMMUNOTHERAPY DC can activate Almost all Immune cells

  6. DENDRITIC CELL BIOLOGY

  7. Dendritic Cell Therapy in Cancer

  8. DEVELOPMENT OF DENDRITIC CELLS Our cells of Interest

  9. WHY DENDRITIC CELLS? There ability to migrate through tissue and act on tumors There capacity to activate naïve T cells Antigen Presenting cell

  10. MoA OF DENDRITIC CELLS ASSOCIATED TUMOR KILLING Tumor Antigen Expanding T-Cells Tumor Killing

  11. Clinical Trials with Dendritic Cell therapy

  12. Companies conducting Clinical Trials with Dendritic Cell Therapy

  13. FEW CLINICAL TRIALS WITH DC

  14. INTERPRETING CLINICAL EFFICACY FOR DC CTs • Pre-mature dismissal of therapy is not suggested if ORR is not high for such a therapy • Unrealistic to expect efficient immune responses to eliminate the total tumor burden in a patient with advanced cancer • Analysis of improved survival benefits in randomized studies and long-term follow-up is suggested • Molecular pathways or chemotherapeutics now considered active based not on only ORR but improved survival and/or time to disease progression • A phase III study comparing DC Therapy with standard chemotherapy (DTIC) in melanoma patients showed insignificant ORR in DC arm but post-hoc analysis demonstrated improved survival and performance status in specific phenotype • Clinical Trials results suggest that DC vaccination therapy needs to be tailored for pre-identified cohorts of patients. • Prolonged survival and good quality of life might be considered a therapeutic success

  15. SOME CONCLUSIONS FROM DC CTs • DCs are the critical decision-making cells in the immune response and an attractive target for therapeutic manipulation to enhance otherwise insufficient immune responses to tumor antigens • Complexity of the DC system requires rational manipulation to achieve protective or therapeutic immunity • Further research needed to analyze the immune responses induced in patients by distinct ex vivo generated DC subsets activated via different pathways • Progresses made in the knowledge of DC biology as well as effector/regulatory T cell biology clearly open the avenues for development of considerably improved clinical protocols • Possibility of including therapeutic vaccination of metastatic disease and preventive vaccination in patients with resected tumors • The ultimate ex vivo-generated therapeutic DC vaccine will be heterogeneous and composed of several subsets, each of which will target a specific immune effector. • These ex vivo strategies should help to identify the parameters for DC targeting in vivo, which lead to the next step

  16. Apceden™

  17. WHAT IS Apceden™ • Apceden™ is an autologous (self) monocyte derived Dendritic Cell immunotherapy which nurtures the patient’s own mononuclear cells against cancer specific cells • Patients undergo apheresis for collection of blood monocytes. These cells are cultured and processed for the production of mature dendritic cells (DC) against the specific tumor cell type, which are harvested on Day 8. • Each dose of APCEDEN™ consists of dendritic cells (CD 80+, 83+, 86+,CD14-) more than 1 million in 15ml • 6 doses are given every 2 weeks for first 3 cycles and next 3 cycles are given every 3 weeks.

  18. Preparation of Apceden™ from Monocytes (1) Incubate for 4-6 Hrs Wash with PBS thrice Adherent cells Buffy Coat Incubate for 6 Days Incubate for 48 Hrs

  19. Preparation of Apceden™ from Monocytes (2) Generation of Immature Dendritic Cells Isolation of Monocytes from peripheral blood Lysate Preparation Loading of Dendritic cells with whole cell Tumor Lysate Isolation of Tumor cells Patient Mature antigen presenting Dendritic Cells The APCEDENTM is to be administered to the patient intravenously

  20. Associated Logistics NutriprepTM Registration 8 Days

  21. APCEDEN™ Dendritic Cells Salubrious Autologous Target Specific Safe Minimal Toxicity

More Related