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Integration of Novel Social Challenges in Medical Biotechnology Education in Hungary

This document discusses the incorporation of emerging social challenges faced by the European Union into the teaching materials of Medical Biotechnology master’s programs at the University of Pécs and the University of Debrecen. Focus is placed on three-dimensional tissue cultures and tissue engineering, covering aspects such as cell-scaffold interactions, growth factors, and the use of Matrigel as a biomaterial. The importance of adapting educational resources to reflect societal needs is emphasized, fostering an innovative learning environment for students in the field of biotechnology.

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Integration of Novel Social Challenges in Medical Biotechnology Education in Hungary

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  1. Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat theUniversity of Pécs and at the University of Debrecen Identificationnumber: TÁMOP-4.1.2-08/1/A-2009-0011

  2. Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat theUniversity of Pécs and at the University of Debrecen Identification number: TÁMOP-4.1.2-08/1/A-2009-0011 Dr. Judit Pongrácz Threedimensionaltissuecultures and tissueengineering – Lecture 11 Cell-Scaffoldinteraction

  3. ScaffoldsI • Pre-made network of biomaterial • One cell type seeded on one scaffold • Several cell types seeded on a scaffold simultaneously or sequentially

  4. ScaffoldsII Fleece Fusedbeeds CO2 Fusedirregular Fusedcubeporogen Cubeporogen

  5. Cell line cells nestling on a scaffold A549 (lung adenocarcinoma cell line)

  6. Matrigel® • Matrigel: • is the trade name for a gelatinous protein mixture secreted by mouse sarcoma cells • This mixture resembles the complex extracellular environment found in many tissues • Matrigel is marketed by BD Biosciences

  7. Primary SAEC(Small Airway Epithelial Cells) in matrigel

  8. Manifestation of Novel Social Challenges of the European Unionin the Teaching Material ofMedical Biotechnology Master’s Programmesat theUniversity of Pécs and at the University of Debrecen Identification number: TÁMOP-4.1.2-08/1/A-2009-0011 Dr. Judit Pongrácz Threedimensionaltissuecultures and tissueengineering – Lecture 12 Biofactors

  9. Biofactors Adjacentcell Wnt Wnt Cytosol Frizzled FGF GSK3 BMP Disheveled FGF receptor b-TrCP b-catenin accumulation b-catenin degradation PI3K TGF-b PLCg Nucleus Jaggedor Delta-like ligands BMP and TGF-breceptor TCF SMAD ATF2 Elk1 TAK Hey ATF2 c-fos c-jun Notch Proliferation Differentiation Migration ICD HES

  10. Main growthfactors (GFs) used in tissue engineering • VEGF– vascularendothelialgrowthfactor • KGF – keratinocytegrowthfactor • TGF-b– transfrominggrowthfactorbeta • BMP– bonemorphogenic protein • FGF– fibroblastgrowthfactor • PDGF– platelet-derivedgrowthfactor

  11. VEGF • Primarilyinendothelialcells • Dimericglycoprotein • Foursplicevariants • Receptors: VEGFR-1 and VEGFR-2

  12. TGF-b • Superfamilymember • MW 21 kDa • Synthesizedbyplatelets and macrophages • Inducesdifferentiationinmultiplecelltypes of varioustissues

  13. BMP • Osteoinductivepeptidefamily • BelongstotheTGF-bsuperfamily • MW 25-30 kDa

  14. FGF • 23 members of thefamily • Multiplefunctionsonmultiplecelltypes • Human FGF-2 18 kDanon-glycosylatedpolypeptidebindstoheparin and heparansulfate

  15. PDGF • Existsas a dimer • Twochains MW 14-17 kDaeach • Synthesizedbyplatelets, macrophages, endothelium, connectivetissue • Effects: Mitogenesis, angiogenesis, macrophageactivation

  16. Delivery of growthfactors • Injected • Diffusedfromscaffolds • Releasedfromgels • Geneticallyengineeredintocells

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