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Agbebi O.T., Alegbeleye W.O., Adebambo O.A., Agbaje M., Odulate D.O ., Olaoye O.J

Characterization, Production and Aquaculture Potentials of Fast Growing Intergeneric Hybrids from Clariid species in Floating Net Bamboo Cage BY. Agbebi O.T., Alegbeleye W.O., Adebambo O.A., Agbaje M., Odulate D.O ., Olaoye O.J . INTRODUCTION.

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Agbebi O.T., Alegbeleye W.O., Adebambo O.A., Agbaje M., Odulate D.O ., Olaoye O.J

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  1. Characterization, Production and Aquaculture Potentials of Fast Growing IntergenericHybrids from Clariid species in Floating Net Bamboo CageBY Agbebi O.T., AlegbeleyeW.O., Adebambo O.A., Agbaje M., Odulate D.O., Olaoye O.J

  2. INTRODUCTION • Aquaculture production in Africa is still insignificant at the global level and accounts for about 0.9 percent the total global aquaculture production in 2000 (FAO, 2003) • Heterobranchus and Clarias species are leading freshwater candidates for aquaculture in Africa, similar in appearance but have differential growth performances in culture. There are many heteroclariasand Clariobranchus types being produced by aquacultural practitioners in Nigeria without paying attention to the type of hybrid fingerlings being released to the ponds or culture medium.

  3. INTRODUCTION CONTD • These F1 hybrids have been reported to be fertile (Aluko, 1998; Nwadukwe, 1995). • The problem posed by this is the indiscriminate use of these fertile hybrids as broodstock for further propagation posing a serious threat to the purity of our indigenous Clariid species. Furthermore, there is need to harmonise the growth records of these hybrids and recommend the fastest growing hybrids. This study was therefore designed to determine the fastest growing F1 hybrids for culture using two Clariid species: H. bidorsalis, and C. gariepinusdomesticated and wild strains

  4. Objectives of the study • Morphological, meristic and molecular characteristics of Clariasgariepinusand Heterobranchusbidorsalisof theparent stocks. • Characterisation of the strains using microsatellite markers • Determination of suitable fish primer for successful taxonomic study • To assess and harmonise various growth records of these hybrids in order to recommend the fastest growing hybrids. • To carry out comparative studies on the desirable characters and strains under the same environmental condition to determine characters that are superior to their parent strains.

  5. MATERIALS AND METHODS • Broodfish Selection and Collection (Wild & Domesticated) • Molecular Characterization -DNA Extraction -Polymerase Chain reaction and -Agarose Electrophoresis • Injection, Fertilization and Incubation Techniques

  6. Experimental Crosses Parental Group • Female H. bidorsalis X Male H. bidorsalis (Domesticated) • Female H. bidorsalis X Male H. bidorsalis(Wild) • Female C. gariepinus X Male C. gariepinus(Domesticated) • Female C. gariepinus X Male C. gariepinus(Wild) Intergeneric Hybrid (Heteroclarias type) • FemaleH.bidorsalis (Domesticated) X Male H. bidorsalis(wild) • Male H. bidorsalis (Domesticated) X female H.bidorsalis (Domesticated) Intergeneric Hybrid (Clariobranchus type) • Female Clariasgariepinus (Domesticated) X male Clariasgariepinus (Wild) • MaleClariasgariepinus (Domesticated) X female Clariasgariepinus (Wild) Intergeneric Hybrid (Domesticated type) • Female Heterobranchusbidorsalis (Domesticated) X Male Clariasgariepinus (Domesticated) • Male Heterobranchusbidorsalis (Domesticated) X female Clariasgariepinus (Domesticated) Intergeneric Hybrid (Wild type) • Male Heterobranchusbidorsalis (Wild) X female Clariasgariepinus (Wild) • Female Heterobranchusbidorsalis (Wild) X male Clariasgariepinus (Wild)

  7. Data collection and analysis • Fecundity (total no of eggs) = 66.6 x female body weight • % Hachability = No. Of hatched eggs x 100 --------------------------No. Of fertilized eggs • % larvae survival = No of normal larvae x 100 -------------------------No. Of hatched eggs

  8. Larval Indoor Rearing • Larval Outdoor Rearing • Two different rearing medium will be used, namely Earthen pond and Cage culture. • 6 Earthen ponds of 10 by 10 metres will be used for the rearing • 12 Cage happas of one cubic metre (1m x1m x 1m) will be constructed. • The cages will be mounted inside the earthen pond simultaneously for growth comparison. • After seven months of rearing in these mediums the skewed sex ratio will be determined to find out the potency of hybridization techniques.

  9. RESULTS AND DISCUSSION • The first grant was utilized only for the purchase of broodstocks and few equipments needed as shown below:

  10. Grand Total = ₦87,500 • Amount Received = ₦87,500 • Amount Spent = ₦87,500 • Balance = ₦00,000

  11. RECOMMENDATION I recommend that second to the fourth grant would be released in order to proceed on the research work on the field. THANK YOU

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