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Gram staining background

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Gram staining background. Gram + Thick cell wall Crystal Violet binds IRREVERDIBLY to the thick peptidoglycan cell wall  purple Gram – Decolorizer (alcohol) washes off the Crystal violet off the Gram – bacteria’s thin cell wall Adding Safranin turns gram – bacteria light pink.

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Gram staining background

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  1. Gram staining background

  2. Gram + • Thick cell wall • Crystal Violet binds IRREVERDIBLY to the thick peptidoglycan cell wall  purple Gram – • Decolorizer (alcohol) washes off the Crystal violet off the Gram – bacteria’s thin cell wall • Adding Safranin turns gram – bacteria light pink

  3. Staining sequence http://highered.mcgraw-hill.com/sites/007337525x/student_view0/exercise9/gram_stain.html Biorad Gram Staining: http://www.cleanvideosearch.com/media/action/yt/watch?videoId=sxa46xKfIOY

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