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Lab # 3 Aseptic Transfer Techniques

Lab # 3 Aseptic Transfer Techniques. Aseptic Transfer Techniques. 1. Protect yourself 2. Protect the environment 3. Maintain pure cultures. Aseptic Transfer Techniques. Aseptic – absence of organisms that may cause contamination or disease Sterile – absence of all life.

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Lab # 3 Aseptic Transfer Techniques

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  1. Lab # 3 Aseptic Transfer Techniques

  2. Aseptic Transfer Techniques • 1. Protect yourself • 2. Protect the environment • 3. Maintain pure cultures

  3. Aseptic Transfer Techniques • Aseptic – absence of organisms that may cause contamination or disease • Sterile – absence of all life

  4. Aseptic Transfer Techniques • 1. Sterile Pipetting • 2. Aseptic Transfer with Inoculating Needle and Inoculating Loop • 3. Alcohol Flaming Technique

  5. TSB - Trypticase Soy Broth • TSA Slant - Trypticase Soy Agar Slant • Turbid – cloudy (10 million bacterial cells per ml) • Sterilize Inoculating Needle and Loop • Always Handle to Tip - never tip to Handle • Prevents dangerous aerosols

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