Download
1 / 1
10 likes | 133 Vues
This study evaluates the percentage of positive HEK293_M2 cells post-lentivirus infection at a multiplicity of infection (MOI) of 0.3 using the pLD-T-hORFeome-IRES-Virus-STOP construct. The analysis covers living cells only, documenting data over varying time points of doxorubicin treatment (12, 24, and 48 hours). Results indicated varying transduction efficiencies, with percentages reaching up to 44% in response to viral infection. Flow cytometry data for forward scatter (FSC-H) and side scatter (SSC-H) provide insight into cell population dynamics.
E N D
Percentage of Venus positive HEK293_M2 cells after lentivirus infection at MOI 0.3 (pLD-T-hORFeome-IRES-Virus-STOP, living cells only) 250 250 250 250 250 250 200 200 200 200 200 200 150 150 150 150 150 150 100 100 100 100 100 100 a 1.7% 1% 1.9% FSC-H FSC-H FSC-H 50 50 50 50 50 50 No virus 0 0 0 0 0 0 0 0 0 0 0 0 101 101 101 101 101 101 102 102 102 102 102 102 103 103 103 103 103 103 104 104 104 104 104 104 SSC-H SSC-H SSC-H 12 hours dox 24 hours dox 48 hours dox b 32% 42% 44% FSC-H FSC-H FSC-H Virus SSC-H SSC-H SSC-H Additional File 5
