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24 hours dox

Percentage of Venus positive HEK293_M2 cells after lentivirus infection at MOI 0.3 (pLD-T-hORFeome-IRES-Virus-STOP, living cells only). 250. 250. 250. 250. 250. 250. 200. 200. 200. 200. 200. 200. 150. 150. 150. 150. 150. 150. 100. 100. 100. 100. 100. 100. a. 1.7%. 1%.

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24 hours dox

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  1. Percentage of Venus positive HEK293_M2 cells after lentivirus infection at MOI 0.3 (pLD-T-hORFeome-IRES-Virus-STOP, living cells only) 250 250 250 250 250 250 200 200 200 200 200 200 150 150 150 150 150 150 100 100 100 100 100 100 a 1.7% 1% 1.9% FSC-H FSC-H FSC-H 50 50 50 50 50 50 No virus 0 0 0 0 0 0 0 0 0 0 0 0 101 101 101 101 101 101 102 102 102 102 102 102 103 103 103 103 103 103 104 104 104 104 104 104 SSC-H SSC-H SSC-H 12 hours dox 24 hours dox 48 hours dox b 32% 42% 44% FSC-H FSC-H FSC-H Virus SSC-H SSC-H SSC-H Additional File 5

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