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Gene Editing Efficiency: DRAP Method for Exon Targeting in Mice and Piglets

Learn about the DRAP method for precise exon targeting in mice and piglets through pronuclear injection and mutant oligonucleotides, achieving efficient gene editing. Discover the implantation and gestation processes, genetic characteristics of the target genes, and the overall efficiency in creating desired mutations.

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Gene Editing Efficiency: DRAP Method for Exon Targeting in Mice and Piglets

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  1. Gene of Interest Exon Target X X DRAP Method Step 1: Pronuclear Injection rDRAP + Mutant Oligonucleotide(s) Fertilized Egg

  2. DRAP Method Step 2: Implantation Mice born in ~ 21 days. Implantation & Gestation Fertilized Egg Piglets born in ~114 days.

  3. DRAP Efficiencies In Mice Gene & Target Characteristics

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