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Methods for Assessing Adipocyte Lipolysis and Glycerol Dynamics in Blood

This document outlines various methods for studying adipocyte lipolysis, including whole-body lipolysis assessment and the rate of glycerol in the bloodstream through isotopic dilution techniques. It discusses potential inconsistencies in measuring regional lipolysis and highlights the use of arteriovenous methods to evaluate lipolytic activity in individual tissues. The document details techniques for measuring glycerol concentrations and blood flow in adipose tissues, as well as various hormone and metabolite measurement methods using high-performance liquid chromatography, radioimmunoassays, and enzymatic approaches.

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Methods for Assessing Adipocyte Lipolysis and Glycerol Dynamics in Blood

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  1. Methods for studying Adipocyte Lipolysis • Whole-Body Lipolysis • Ra of glycerol in the bloodstream • Measuring the dilution of tracer-labeled glycerol in plasma (isotopic dilution) • Has some possible inconsistencies • Regional Lipolysis • Lipolytic activity can be assessed in individual tissues using arteriovenous methods • Evaluation of the balance between delivery and release of glycerol across adipose tissue bed. • Done by measuring arterial & venous glycerol concentrations and adipose tissue blood flow (ATFB) • Methods were arterial and venous catheterization and microdialysis probes. Schematic diagram of a microdialysis probe and the method used to collect samples

  2. More Measurement Techniques • Measurements of hormones, metabolite concentrations and blood lactate concentrations were measured by Goto via different methods • Epi & Ne measured with high performance liquid chromatorograph kits • Serum GH measured with radioimmunossay kits • Serum Insulin measured with Enzymic immunoassay • Serum FFA & Ketone Body measured with Enzymatic methods • Serum Glycerol measured with Enzymatic colorimetric • Lactate measured by pricking a fingertip for a blood sample

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