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Biotechnology and Recombinant DNA

9. Biotechnology and Recombinant DNA. Complementary DNA. Figure 9.9. Recombinant DNA. Figure 9.11 (1 of 2). Figure 9.11, steps 3–5. Recombinant DNA. Figure 9.11 (2 of 2). Making a Gene Product. Figure 9.12 (1 of 2). E. coli. Used because it is easily grown and its genomics are known

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Biotechnology and Recombinant DNA

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  1. 9 Biotechnology and Recombinant DNA

  2. Complementary DNA Figure 9.9

  3. Recombinant DNA Figure 9.11 (1 of 2)

  4. Figure 9.11, steps 3–5

  5. Recombinant DNA Figure 9.11 (2 of 2)

  6. Making a Gene Product Figure 9.12 (1 of 2)

  7. E. coli • Used because it is easily grown and its genomics are known • Need to eliminate endotoxin from products • Cells must be lysed to get product

  8. Therapeutic Applications • Subunit vaccines • Nonpathogenic viruses carrying genes for pathogen's antigens as vaccines • Gene therapy to replace defective or missing genes

  9. RNA Interference (RNAi) Figure 9.14

  10. Scientific Applications • Understanding of DNA • Sequencing organisms' genomes • DNA fingerprinting for identification Figure 9.17

  11. Southern Blotting Used for Genetic Screening Figure 9.16, steps 1–2

  12. Southern Blotting Figure 9.16, steps 3–4

  13. Southern Blotting Figure 9.16, steps 5–6

  14. Agricultural Applications Table 9.2

  15. Result of Agrobacterium tumefaciens infection Figure 9.19

  16. Genetic Modification Using Agrobacterium Figure 9.20

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