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STREPTOCOCCI ENTEROCOCCI

STREPTOCOCCI ENTEROCOCCI. Dr. Irena Hanovcová, PhD. dept. of Microbiology. STREPTOCOCCUS spp. Gram-positive round or lancet-shaped cocci, in chains or pairs nonmotile nonsporing facultative anaerobes catalase - negative. STREPTOCOCCUS spp. division

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STREPTOCOCCI ENTEROCOCCI

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  1. STREPTOCOCCIENTEROCOCCI Dr. Irena Hanovcová, PhD. dept. of Microbiology

  2. STREPTOCOCCUS spp. • Gram-positive round or lancet-shaped cocci, in chains or pairs • nonmotile • nonsporing • facultative anaerobes • catalase - negative

  3. STREPTOCOCCUS spp. division • according production of hemolysis on BA a - hemolyticb - hemolyticg - hemolytic, nonhemolytic • according polysaccharid substance Cdiagnostic schema of Lancefield - 20 serogroups:A,B,C,D,F,G - main human pathogensviridans streptococci - without polysacch. C

  4. S. pyogenes A S. agalactiae B S. dysgalactiae C S. bovis D S. pneumoniae S. salivarius S. sanguis S. mitis S.mutans S. „milleri“ STREPTOCOCCUS spp.

  5. S. pyogenes - serogr. A antigenic structure • capsule hyaluronic acid, nonimmu- nogenic, antiphagocytic • M protein antiphagocytic, anticomple- mentary • F protein adherence to epithel. cells • lipoteichoic a. adherence to epithel. cells

  6. S. pyogenes - serogr. A exoproducts • erythrogenic pyrogenic, delayed hypersen- toxin sitivity, susceptibility to ET, cytotoxic, mitogenic for T ly, immunosupresion of B ly, scarlatiniform rash • streptolysin S nonimmunogenic lyses WBC, RBC, platelets • streptolysin O immunogenic - ASLO action as previous • streptokinase spread f. - lyses blood clots

  7. S. pyogenes - serogr. A infectionspharyngitisscarlet fever erysipelasimpetigopyodermatoxic shock syndromrheumatic feverglomerulonephritis

  8. G + cocci in chains and small clusters(smear prepared from cultivation)

  9. G + cocciin chains from clinical speciment

  10. S. pyogenes gr. Acultivation on BA b-hemolysis

  11. S. agalactiae - serogr. B infectionsneonatal inf. -meningitis pneumonia bacteremiapostpartum sepsis

  12. S. agalactiae gr. Bcultivation on BA, CAMP test S. agalactiae S. aureus

  13. S. pneumoniae • diplococci of lancet shape • without polysaccharide C • polysaccharide capsule - antiphagocytic growth phases - M, S, R • infections - pneumonia sinusitis, otitis media meningitis bacteremia

  14. S. pneumoniaesmear from CSF

  15. S. pneumoniaesmear from sputum

  16. S. pneumoniaecultivation on BA with optochin

  17. viridans streptococci • without polysaccharide Ca- or nonhemolytic • some producers of polysaccharides (dextran) • normal flora of oropharynx • infections - bacteremia endocarditis dental caries

  18. STREPTOCOCCUS spp. • laboratory dg • microscopy - from all materials instead of swabs from oropharynx • cultivation - BA • bacitracin test + gr.A • CAMP test+ gr.Bhydrolysis of hippurate + • hydrolysis of esculin gr.D • optochin sens., bile soluble S. pneumoniae • latexagglutination

  19. STREPTOCOCCUS spp. • treatmentpenicillin Gmacrolides • preventionrheumatic fever - depot penicillin endocarditis - amoxicillinpneumococcal vaccine

  20. ENTEROCOCCUS spp. • formely streptococci of serogroup D • G+ cocci in pairs or chains • resistant to higher concentrations of saltand bile • habitants of GIT S. faecalisS. faecium • often polyresistant (PNC, AMG, vancomycin)

  21. ENTEROCOCCUS spp. • infectionsUTIendocarditisintra-abdominal, pelvic inf. • treatmentaccording the results of sensitivity testsendocarditis - PNC + GEN

  22. ENTEROCOCCUS spp.bile - esculin test

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