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Preliminary Results

Preliminary Results. T. Erin Cox xFOCE seminar 19 November 2013. Installation in May. 1m. Control . 2m. Reference . Experiment. Reference = R Control = C Experiment = E. Reference = R Control = C Experiment = E. Shiny used to monitor sensor data .

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Preliminary Results

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  1. Preliminary Results T. Erin Cox xFOCE seminar 19 November 2013

  2. Installation in May 1m Control 2m Reference Experiment Reference = R Control = C Experiment = E Reference = R Control = C Experiment = E

  3. Shiny used to monitor sensor data http://shiny.obs-vlfr.fr/efoce/datavis/ Developed by Jean-Pierre & Alice Webb with input from eFOCE members

  4. Logistics of Sampling

  5. P. oceanica abundance & density Example- Shoot density (mean ±SE) Reference = R Control = C Experiment = E

  6. P. oceanica shoot variables Example- Leaf blade length (mean ±SE) Reference = R Control = C Experiment = E

  7. P. oceanica biomass Relationship can be used to estimate biomass in chambers or plot Reference = R Control = C Experiment = E

  8. Incubations • We performed 3 types of incubations • 1. Whole Community – entire chamber incubated • 2. Seagrass – shoot enclosed • 3. Sediment – sediment with rhizomes enclosed • Changes in O2, Dissolved Inorganic Carbon, & Total Alkalinity were measured • To determine impacts to production, respiration, and calcification rates

  9. Tested many designs 3 incubation bags per chamber or plot 4 hrs light & 8 hrs darkness Collected shoot to express as dry weight Seagrass Shoot Incubations

  10. Sediment Incubations Tested many designs 1 fixed location per chamber or plot, over 3 days (n = 3) 24 hrs incubation

  11. Reference = R Control = C Experiment = E Variable Net Production Seagrass Sediment n = 1 – 3 (mean ±SE) Treatments x Month

  12. Whole community incubations Photosynthesis vs Irradiance curves

  13. Pulse Amplitude Modulation (PAM)Ability to Harvest Light Energy in P.oceanica n = 5 – 6 (mean ±SE) Reference = R Control = C Experiment = E

  14. Rapid Light Curve Parameters n = 5 – 6 (mean ±SE) Reference = R Control = C Experiment = E

  15. Other data collection • Sediment for • Diversity of microbes • Pigments • Mineralogy • Community of Foraminifera • C/N content isotopic ratios • Seagrass for • Calcified Epiphyte Mass • Epiphyte community abundance & diversity • Phenolics • DMSP • C/N content & isotopic ratios • Benthic profiler • Settlement & Recruitment Tiles

  16. Relocated Sites in November • 5 months of data collection • Repeated physical disturbance from sampling & installing electronics • Intense & repeated collections • Site no longer representative of the meadow

  17. Future Actions • Eliminate all nets • Dive tanks no longer sit on benthos • Eliminate the sediment & seagrass incubations • Reduce the collection (# & frequency) of leaves & sediment

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