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High Risk Autopsy

High Risk Autopsy. Randal R. Nixon, MD PhD Oregon Health & Sciences Univ. Dept. of Pathology, Portland, OR Cleveland, OH - 6/23/04. Source Material. High Risk Autopsies. Case Selection Permissions Local NPDSU (required for genetic testing) Precautions Autopsy - BSL 2 Tissue - BSL 3

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High Risk Autopsy

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  1. High Risk Autopsy Randal R. Nixon, MD PhD Oregon Health & Sciences Univ. Dept. of Pathology, Portland, OR Cleveland, OH - 6/23/04

  2. Source Material

  3. High Risk Autopsies • Case Selection • Permissions • Local • NPDSU (required for genetic testing) • Precautions • Autopsy - BSL 2 • Tissue - BSL 3 • Equipment • Procedure • Tissue Handling • Shipping

  4. Limit Exposure Respirator Vacuum Limited personnel Limit Contamination Disposables Shroud Coverings Dissection container Dedicated tool set High Risk Autopsies - Goals . Obtain diagnostic tissue

  5. High Risk Autopsies - Equipment • HEPA Respirator suit (Enviro-Safety Products) http://envirosafetyproducts.com • HEPA Vacuum (Mar-Med Inc.) 1-440-572-5175 • Disposables (Mopec) • Cutting board, large • Brain Knife • Headrest • Plastic Tub • Biohazard Bags

  6. Stainless steel Autoclavable 1M NaOH 30’ Non-autoclavable Immersible 1-2M NaOH, 1hr, RT Non-Immersible 1-2M Aluminum Sodium hypochlorite (2% free chlorine) DO NOT AUTOCLAVE Liquid waste 1M NaOH Autoclave 132°, 4.5hr Dry waste Incineration Autoclave 132°, 4.5hr High Risk Autopsies - Decontamination

  7. Tissue Processing • Post-fixation Formic Acid • >95%, 1hr, RT with post-treatment formalin 48hr. • Process as usual • Discard waste as biohazardous waste

  8. Shipping Infectious substance, 6.2 UN 1 fiberboard box 602 Affecting humans 2814 X 10g (Prions, PrP-Sc) Dry Ice 9 UN III 10kg 904 1845 OVERPACK USED Emergency contact: NAME / PHONE NUMBER Prior arrangements as required by the IATA Dangerous Goods Regulations 1.3.3.1 have been made.

  9. Useful Websites • CDC CJD • http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4s7d.htm • WHO TSE • http://www.who.int/emc-documents/tse/whocdscsraph2003c.html • WHO Shipping • www.who.int/emc/pdfs/emc97_3.pdf • HEPA Respirator • http://envirosafetyproducts.com

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