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Characterisation of the comABCDE pathway in S. pneumoniae

Characterisation of the comABCDE pathway in S. pneumoniae. Megan Aylward. 19/06/08. Overview. Background What comABCDE regulates When it is switched on/off Possible negative feedback. Overview. Components. Characterisation of the promoter region. Difficulties in finding the information

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Characterisation of the comABCDE pathway in S. pneumoniae

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  1. Characterisation of the comABCDE pathway in S. pneumoniae Megan Aylward 19/06/08

  2. Overview • Background • What comABCDE regulates • When it is switched on/off • Possible negative feedback

  3. Overview

  4. Components

  5. Characterisation of the promoter region • Difficulties in finding the information • ComX with RNA polymerase haloenzyme binds to cin-box –tacgaata • Strength information not available

  6. Identification of promoter region • BLAST analysis against S. pneumoniae genome • Clustal alignment • Tried to find the promoter region in the genome cin-box ------------------------------TACGAATA---------------------- section CGAATAATTCTTTTCTATTTATTTGACCTTTACAAATAAAATGGTAACTGTGACTAATAA embl|U76218|U76218 AAAAGGATTTTCTACTCTTACTTTTATTATTG-GGGGAAGTTTAGGATTGTCATCATCTG

  7. Model • Karlsson et al (2007) • Variables within their model include;

  8. Parameters • ComCDE- Basal synthesis, maximal synthesis, ComE-P conc. required • All components are assumed to undergo natural decay • Some parameters from literature where available

  9. Modelling in COR :there is one or several problems with units in this equation

  10. Running COR ComD model Problem with the CVODE integrator: at t=0 and h=2.18217e-0.15, the corrector convergence test failed repeatedly or with |h|=hmin.

  11. Future Modelling • Draft COR model for ComD has been generated • Other components need more information • Learn differential equations

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