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Step-Up Year 1 Practical Laboratory Session: Thin Layer Chromatography

Step-Up Year 1 Practical Laboratory Session: Thin Layer Chromatography. TLC. Chromatography is a separation method with 2 phases; 1. Mobile 2. Stationary. http://www.aquaculture.ugent.be. TLC. Benefits of Thin Layer Chromatography Only need a small amount of sample

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Step-Up Year 1 Practical Laboratory Session: Thin Layer Chromatography

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  1. Step-Up Year 1 Practical Laboratory Session: Thin Layer Chromatography

  2. TLC Chromatography is a separation method with 2 phases; 1. Mobile 2. Stationary http://www.aquaculture.ugent.be

  3. TLC Benefits of Thin Layer Chromatography • Only need a small amount of sample • Fast and inexpensive (relatively) • Can identify different components of a mixture by separating them

  4. Experimental Procedure • Marking Mark the plate lightly with a pencil • Touching the plateFingerprints • ‘Spotting’ Small and well spaced – best resolution • Safety

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