Enhancer Activity of Novel Hoxa9/Meis1 Binding Regions in Lucerne Assays
This study investigates twenty-two novel binding regions for Hoxa9 and Meis1, each approximately 1,000 bp long, which were cloned into the pTAL-luc luciferase reporter and electroporated into K-562 cells. The enhancer activities of these regions were compared to a known Runx1 conserved noncoding element (Runx1_chr16(+23)) and two randomly selected control regions. Results are presented as Mean ± SD in triplicate, normalized to pTAL-luc, with statistical significance determined by standard t-test.
Enhancer Activity of Novel Hoxa9/Meis1 Binding Regions in Lucerne Assays
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Presentation Transcript
** ** *** ** *** ** Figure S2. Novel Hoxa9/ Meis1 binding regions show enhancer activity in luciferase assays. Twenty-two novel Hoxa9/ Meis1 binding regions of ~1,000 bp in length were cloned into pTAL-luc luciferase reporter and electroporated into K-562 cells. Enhancer activity of the Hoxa9/ Meis1 binding regions was compared to a known Runx1 conserved noncoding element(Runx1_chr16(+23)) and two control regions randomly selected in the genome as a negative control. Data is presented as the Mean +/- SD in triplicate normalized to pTAL-luc. ([*] P > 0.05; [**] P < 0.05; [***] P < 0.001) P values determined using Standard t test.
