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JNK Activation in Dopaminergic Neurons Modulated by siRNA to Jnk1 or Jnk2

This study investigates the attenuation of JNK activation in dopaminergic neurons induced by paraquat or rotenone through siRNA targeting Jnk1 or Jnk2. Primary E14 mesencephalic neurons were transfected with siRNA and treated with toxins. Quantification of p-JNK levels in TH+ neurons was performed. Results show partial attenuation with significant statistical relevance.

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JNK Activation in Dopaminergic Neurons Modulated by siRNA to Jnk1 or Jnk2

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  1. A * ns B *** * Supplemental Figure 1. Paraquat- (A) or rotenone- (B)-induced JNK activation in dopaminergic neurons is partially attenuated by siRNA to Jnk1 or 2. Primary cultured E14 mesencephalic neurons were transfected with Jnk1 or 2 siRNA or control siRNA. Twenty-four hours after transfection, cells were treated with 40 µM paraquat, 5 nM rotenone or their respective vehicle controls for 8 h. Cells were fixed and stained for TH and p-JNK. The intensity of JNK phosphorylation in TH+ dopaminergic neurons was quantified. *p<0.05; ***p < 0.005; ns, not statistically significant.

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