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András G ÁSPÁRDY , István A NTON , István K OMLÓSI , László N AGY , László S ÁFÁR and Imre B ODÓ

Ann. Meet. of DAGENE, Bled, Slovenia, 03.09.2004. Application of DNA-markers to distinguish Tsigai sheep variants. András G ÁSPÁRDY , István A NTON , István K OMLÓSI , László N AGY , László S ÁFÁR and Imre B ODÓ. Transylvanian ancient type. Main Tsigai variants. North Carpathians

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András G ÁSPÁRDY , István A NTON , István K OMLÓSI , László N AGY , László S ÁFÁR and Imre B ODÓ

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  1. Ann. Meet. of DAGENE, Bled, Slovenia, 03.09.2004.Application of DNA-markers to distinguish Tsigai sheep variants András GÁSPÁRDY, István ANTON, István KOMLÓSI, László NAGY, László SÁFÁR and Imre BODÓ

  2. Transylvanian ancient type

  3. Main Tsigai variants North Carpathians mountain ecotype (Jákotpuszta) Great Plain lowland ecotype Transylvania (Kardoskút, Akasztó, Makó) Southland milking type (Cegléd)

  4. Mountain ecotype

  5. Main Tsigai variants North Carpathians mountain ecotype (Jákotpuszta) Great Plain lowland ecotype Transylvania (Kardoskút, Akasztó, Makó) Southland milking type (Cegléd)

  6. Lowland ecotype

  7. Main Tsigai variants North Carpathians mountain ecotype (Jákotpuszta) Great Plain lowland ecotype Transylvania (Kardoskút, Akasztó, Makó) Southland milking type (Cegléd)

  8. Milking type

  9. Previous knowledge • breed history, • conformation, • wool production, • milk production, • mutton production • biochemical polymorphism

  10. Blood samples, 5, 48 2003 Eight microsatellites PCR (Perkin Elmer DNA termal cycler) ABI PRISM 310 genetic analyzer GENEPOP population genetics software package Hardy-Weinberg equilibrium Genotype and allelic frequencies Shannon’s information index Heterozygosity statistics Wright’s fixation index Nei’s genetic identity and distance Materials and methods

  11. Result IGenic variation statistics (mean)

  12. Result IIHeterozygosity statistics (mean)

  13. Result IIIWright’s fixation index

  14. Result IVDendrogram according to Nei’s genetic distances

  15. Result IVDendrogram (Nei’s genetic distances) • North Carpathians • mountain ecotype (Jákotpuszta) • Great Plain • lowland ecotype Transylvania • (Kardoskút, Akasztó, Makó) • Southland • milking type (Cegléd)

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