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Lab # 9 Photosynthesis

Lab # 9 Photosynthesis. Purpose. To study and learn the properties of photosynthesis. Background Information. Photosynthesis , is the critical link between the energy of the sun and the food and fuel we consume in our daily lives.

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Lab # 9 Photosynthesis

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  1. Lab # 9 Photosynthesis

  2. Purpose • To study and learn the properties of photosynthesis.

  3. Background Information • Photosynthesis, is the critical link between the energy of the sun and the food and fuel we consume in our daily lives. • When plants photosynthesize they release oxygen into the atmosphere. • This oxygen comes from water in the cells of leaves and is initially released into spaces inside leaves.

  4. Background Information • The oxygen then moves from leaves into the atmosphere through small holes on the leaf surface called stomata (singular: stoma). • In this exercise the production of oxygen is used as a measure of the rate of photosynthesis. • Plants also need carbon dioxide for photosynthesis. • For this investigation carbon dioxide is provided by a baking soda solution.

  5. Hypothesis • If plants are placed in light then they will produce _____________. • If plants are placed in the dark then they will not produce _____________.

  6. Materials • Baking soda + water • Syringe • Spinach leaf • Lamp • straw

  7. Procedure • Using a straw punch out 5 disks from a young spinach leaf. • Pull the plunger out of the syringe. Place the leaf disks into the syringe and replace the plunger. • Draw up 8 ml of baking soda solution into the syringe. Invert the syringe, tip end up, and gently push the plunger to remove all the extra air. • Put your finger over the syringe tip and pull the plunger. This will create a vacuum which will pull the air and oxygen from the leaf discs.

  8. Procedure • Repeat until all of the discs sink. Do steps 1 to 5 for two syringes. • Place one syringe narrow-end up about 5 cm from the lamp. Place the second syringe in the dark. • As the leaf discs photosynthesize and produce oxygen, they will float to the top. Record the time at which each disc floats. You may have to tap the syringe every 20 to 30 seconds.

  9. Data Calculate the average time for light and dark.

  10. Conclusion • Summarize the lab activity. • What caused the spinach discs to float when placed under the lamp? • Did the disks float in the dark? • Why was baking soda added to the water? • What is photosynthesis?

  11. Conclusion • What are stomata? • What is cellular respiration? • What is the formula for photosynthesis? • What factors can affect the rate of photosynthesis? • What type of organisms can undergo photosynthesis?

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