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Mosquito Larvicidal Activity of Ethanolic Extracts from Cryptomeria japonica

This study evaluates the larvicidal activity of ethanolic extracts from leaves, wood, and bark of Cryptomeria japonica against Aedes aegypti and Aedes albopictus fourth-instar larvae. Effective concentrations were measured as LC50 and LC90 after 24 and 48 hours of treatment. Results indicated that the wood extract of C. japonica showcased significant larvicidal properties, providing 100% mortality in A. aegypti and substantial mortality in A. albopictus. These findings suggest potential for developing biodegradable and natural larvicidal agents from C. japonica.

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Mosquito Larvicidal Activity of Ethanolic Extracts from Cryptomeria japonica

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  1. Hui-Jing GU IUFRODivision 5 Conference 5.07.00 D Forest Products / Energy and chemicals from forest biomass / Chemicals from wood

  2. Hui-Jing GU, Sen-Sung CHENG, Hui-Ting CHANG and Shang-Tzen CHANG School of Forestry and Resource Conservation, National Taiwan University No. 1, Section 4, Roosevelt, Taipei, Taiwan, ROC Tel +886-2-3366-4614; Fax +886-2-3365-4520 Mosquito larvicidal activity of ethanolic extracts of Cryptomeria japonica

  3. Larvicidal activity was evaluated after 24 h and 48 h treatments. Effectiveness were reported as LC50 and LC90. Aim of work and methods The purpose of this study is to determine the larvicidal activities of ethanolic extracts from leaves, wood and bark of Cryptomeria japonicaagainst fourth-instar larvae of the mosquitoes Aedes aegypti and Aedes albopictus. Plant material Mosquito larvae • Leaf • Bark • Wood Aedes aegypti Aedes albopictus Cryptomeria japonica

  4. Results Cryptomeria japonica Extraction Wood extract A. aegypti（ LC50 ＝63.2 μg/mL ） A. albopictus（LC50＝93.8 μg/mL ） Bioassay Extracts Liquid-Liquid partition n-Hexane A. aegypti（ LC50 ＝ 36.5 μg/mL ） A. albopictus（LC50＝111.8 μg/mL ） Fractions Bioassay CC, TLC H5 A. aegypti（ 100% mortality ） A. albopictus （72.5% mortality ） Bioassay Subfractions HPLC epi-Cubebol Cubebol Bioassay Compounds IR, UV, MS, NMR

  5. Conclusions This study demonstrated that the ethanolic extract of C. japonica wood and its major compounds have excellent mosquito larvicidal activities against both A. aegypti and A. albopictus. These result could be useful in the research for selecting newer, more selective, biodegradable and natural larvicidal agents. Thank You for Your Attention!

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