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The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract

The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract . by Niel Henriksen Mentor: Dr. Phil Proteau College of Pharmacy. Hawthorn and Heart Failure.

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The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract

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  1. The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract by Niel Henriksen Mentor: Dr. Phil Proteau College of Pharmacy

  2. Hawthorn and Heart Failure • Congestive heart failure (CHF) results from coronary artery disease, a previous heart attack, high blood pressure, heart defects, and infections in the heart tissue. • Nearly 5 million people in the United States are diagnosed with CHF. • The symptoms of CHF include shortness of breath, fatigue, swelling and kidney dysfunction.

  3. Hawthorn Extract Benefits • Clinical trials by the NYHA demonstrated: (+) exercise tolerance (-) dyspnea (shortness of breath) (-) ankle swelling (-) blood pressure • Studies on cardiac tissue show: (+) inotropic effects (+) cardiac flux (+) vasorelaxation (+) anti-arrhythmic potential

  4. Hawthorn Extract Unknowns • Active compound(s) • Flavonoids and Procyanidins? • Mechanism of action • Proper dosing • Duration of therapy • Most effective extract source (berries, leaves, or flowers)

  5. Project Goals • Develop cell based bioassays for evaluation of the direct cardiac effects of hawthorn extract • Using bioassay-guided fractionation, identify the components in hawthorn extract with cardiac activity

  6. Bioassay Tests • Rate of Contraction • Amplitude of Contraction

  7. Isolation Techniques • Solvent Extraction • Thin-Layer Chromatography • Large Scale Chromatography • High Performance Liquid Chromatography

  8. Identification Techniques • Mass Spectrometry • Nuclear Magnetic Resonance (NMR) Spectroscopy

  9. Future Goals • Complete bioassay-guided fractionation • Determine the most effective extract source • Determine the mechanism through with hawthorn extract functions • Correlate the plasma levels of cardioactive component with the physiologic effects on animal models

  10. Acknowledgements • Dr. Phil Proteau • Shannon Long • Dr. Theresa Filtz • Dr. Kevin Ahern • Howard Hughes Medical Institute

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