1 / 9

Mapping leaf rust resistance genes in wheat

Mapping leaf rust resistance genes in wheat. Jim Kolmer USDA-ARS Cereal Disease Laboratory Jim Anderson Univ. of Minnesota. Thanks to Kun Xiao, Jen Flor. RL6149. RL6149 = Thatcher*6/T. turgidum 8404 Developed by P. Dyck RL6149 IT is ; (fleck) 20-50 MRMS Thatcher x RL6149 F 2

linore
Télécharger la présentation

Mapping leaf rust resistance genes in wheat

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mapping leaf rust resistance genes in wheat Jim Kolmer USDA-ARS Cereal Disease Laboratory Jim Anderson Univ. of Minnesota Thanks to Kun Xiao, Jen Flor

  2. RL6149 RL6149 = Thatcher*6/T. turgidum 8404 Developed by P. Dyck RL6149 IT is ; (fleck) 20-50 MRMS Thatcher x RL6149 F2 (: - 3) 100 (3+4) 30 F3 families 32 HR 63 Seg 25 HS

  3. RL6149 F3 families used for mapping 6AL location – flanked by Barc104 (13.9cm) and GWM427 (21 cm) No other Lr gene on 6AL Designated as Lr64

  4. RL6062 RL6020 = Thatcher*6/ PI268316 Developed by P. Dyck PI268316 common wheat RL6062 22+ IT 30-50 MRMS Thatcher x RL6062 F2 (2-2+ ) 90 (3-4) 43 F3 families 26 HR 73 SEG 36 HS Infection type F2

  5. RL6062 Polymorphic SSR markers not linked to resistance gene Bulk Segregation Analysis 15 HR 15 HS F3 families 8 DArT markers on 6BL in disequilibrium – distal region SSR data Lr3 - Lr9 – linkage? RL6062 isolate specificity different P. L. Dyck - 1980

  6. Americano 25eUruguay landrace derived1918 – highly resistant Tc*2/Americano 25e F6 Adult plant resistance – no seedling resistance 10-20 MRMS field resistance 0;2- adult plant IT greenhouse test Tc*3/ Americano 25e F6 lines Three field tests – greenhouse test 1-2 genes segregating Severity (%)

  7. Tc*3/Americano 25e F6 lines DArT map Major effect on 1BL – likely Lr46 Select Tc*3 lines with Lr46 only

  8. Tc*3/Americano 25e F6 lines Smaller effect on 5B No effect St Paul 2010 (fewer high severity lines) Select Tc*3 lines with 5B QTL

  9. Tc*3/Americano 25e F6 linesR2 values

More Related