120 likes | 359 Vues
Population genetics of the wheat leaf rust fungus, Puccinia triticina in Central Asia. J. A. Kolmer M. E. Ordonez USDA-ARS Cereal Disease Laboratory University of Minnesota -St. Paul. Leaf Rust Collections. Collections from 2002, 2004, 2005 A. Morgounov - CIMMYT, Almaty Kazakhstan
E N D
Population genetics of the wheat leaf rust fungus, Puccinia triticinain Central Asia J. A. Kolmer M. E. Ordonez USDA-ARS Cereal Disease Laboratory University of Minnesota -St. Paul
Leaf Rust Collections • Collections from 2002, 2004, 2005 • A. Morgounov - CIMMYT, Almaty Kazakhstan • 137 isolates tested for virulence - 99 for SSR • Caucasus - (Azb.,Geo. Arm.) 13 Uzbekistan - 7 Tajikistan-Kyrgyzstan -15 S. Kazakhstan - 39 N. Kazakhstan- 25 N. America-16 Durum - 2 Clone corrected within populations (race/SSR genotype) 20 differentials - 23 SSR loci Super highway - Kazakhstan
Tjk-Kyg-Uzb.-Caucasus not sig. differentiated S. Kaz - N. Kaz.not sig. differentiated Differentiation Statistics (Phi) of Pair-wise Populations - Virulence phenotypes Neighbor joining
Differentiation (Fst) Statistics of Pair-wise Populations SSR Genotypes Tjk-Kyg-Uzb - Caucasus not signif. differentiated S. Kaz - N. Kaz. not signif. differentiated 0.82 correlation with virulence tree Neighbor joining
Individual Isolates SSR Allele Variation -Central Asia 1 Avir Lr2a Vir Lr2c 2 4 Most from Cauc-Tjk-Kry-Uzb. 3 Vir Lr2a Vir Lr2c Most from S.+ N.Kazakhstan -North America Avir Lr2a Vir Lr2c Vir Lr2a Lr2c Avir Lr2a Lr2c -Durum 6 Avir. to almost all Lr genes Neighbor joining
Central AsiaP. triticina populations Come to Kazakhstan!!! • Two distinct regional populations - mountain barrier • Limited migration between regions - very few SSR-virulence genotypes in common • Strong relationship between virulence and SSR genotypes
Population Genetics of P. triticina - Future Goals • Describe world-wide populations 20 Tc line differentials 23 SSR loci • North America, Central Asia, Middle East, South America, Europe • Determine genetic relationships between populations - (MCDS - USA- S. America)