1 / 7

Lab 2: Organelles, Proteins, and SDS-PAGE

Lab 2: Organelles, Proteins, and SDS-PAGE. Dr. Sarah VanVickle -Chavez BIO 2960 Laboratory Spring 2013. Overview . Today we will load a gel and compare your gel to the sample gel. Each person will load one lane on the gels in the center of each bench.

livia
Télécharger la présentation

Lab 2: Organelles, Proteins, and SDS-PAGE

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Lab 2: Organelles, Proteins, and SDS-PAGE Dr. Sarah VanVickle-Chavez BIO 2960 Laboratory Spring 2013

  2. Overview • Today we will load a gel and compare your gel to the sample gel. Each person will load one lane on the gels in the center of each bench. • While gels are running, we will do the PyMOL computer lab as a group demo. You should have Lab Report 1a complete before we leave today. • You can use your books and notes, but there is a brief computer tutorial on the NSLC website to help you with Lab Report 1b. • http://www.nslc.wustl.edu/courses/Bio2960/labs.html • Next week, there are three reports due at the beginning of class • 1a = proteins (PyMOL demo in class) • 1b = cell organelles (use your book/notes for this one) • 2 = SDS-PAGE

  3. 1. Protein Electrophoresis • SDS-PAGE stands for sodium dodecyl sulfate polyacrylamide gel electrophoresis

  4. SDS vs Native Gel How do you “see” your proteins?

  5. PyMOL • Remember: Amino acids have different properties based on the R-group • GAVLIMPFW = nonpolar • STNQYC = uncharged, polar • KRHDE = charged, polar

  6. Alpha helix

  7. Beta Sheet

More Related