1 / 2

Ad5 Coated Ad5

Polymer-coated adenovirus are protected against neutralizing antibodies Suzie H. Pun, University of Washington, DMR 0706647. Overall Goal: Develop a polymeric coating that self-assembles with and potentiates adenoviral ( AdV ) vectors.

liza
Télécharger la présentation

Ad5 Coated Ad5

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Polymer-coated adenovirus are protected against neutralizing antibodiesSuzie H. Pun, University of Washington, DMR 0706647 Overall Goal: Develop a polymeric coating that self-assembles with and potentiates adenoviral (AdV) vectors. Results summary: We synthesized and evaluated 10 different peptide-polymer materials for their ability to coat AdV and increase transduction efficiency to CAR-negative cells. The optimal polymer was able to increase the % transduced cells by up to 6-fold in three different cell lines. Top Figure: Schematic of virus coating with branched peptide-HPMA copolymer. Middle figure: TEM images of virus and coated virus. Bottom figure: Polymer coating protects adenovirus from neutralizing antibodies. This transduction was conducted in CHO-K1 CAR-negative cells. Ad5 Coated Ad5

  2. Spatially-controlled immobilization of polyplexesSuzie H. Pun, University of Washington, DMR 0706647 The Pun Laboratory presented for University of Washington’s Math Academy, a program that supports 36 high school juniors from underrepresented minority backgrounds in a 4-week residential session at UW. The Pun lab prepared lectures and several hands on demonstrations.

More Related