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Replication Study: Controls dH2O Instead of Caffeine

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This study replicates results using controls of dH2O on disk rather than caffeine. The experiment includes plates and gels with specific picks and origins PCR to compare high-high vs. low-low replicates. Chaperone PCR gel analyses for polyacrylamide and agarose repeats are also conducted.

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Replication Study: Controls dH2O Instead of Caffeine

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Presentation Transcript

  1. Replication Results By: Erica Keffeler and Jesse Campbell

  2. Controls dH2O on Disc Instead of Caffeine Amp ONLY Plate/ No Tet

  3. Plate 4 High-High V.S. Low-Low

  4. Plate 5 High-High V.S. Low-Low

  5. Plate 6 High-High V.S. Low-Low

  6. Origin PCR Origin PCR Plate 4 Gel 1 Picks 1-11 Gel 2 Picks 12-22 Gel 3 Picks 23-33 Gel 4 Picks 34-44 Gel 5 Picks 45-55 Gel 6 Picks 56-66

  7. Origin PCR Origin PCR Plate 4 Gel 7 Picks 67-77 Gel 9 Picks 89-96 Gel 8 Picks 78-88

  8. Origin PCR Origin PCR Plate 5 Gel 1 Picks 1-11 Gel 2 Picks 12-22 Gel 3 Picks 23-33 Gel 4 Picks 34-44

  9. Origin PCR Origin PCR Plate 5 Gel 5 Picks 45-55 Gel 6 Picks 56-66 Gel 7 Picks 67-77 Gel 8 Picks 78-88 Gel 9 Picks 89-96

  10. Origin PCR Origin PCR Plate 6 Gel 1 Picks 1-11 Gel 2 Picks 12-22 Gel 3 Picks 23-33 Gel 4 Picks 34-44

  11. Origin PCR Origin PCR Plate 6 Gel 5 Picks 45-55 Gel 6 Picks 56-66 Gel 7 Picks 67-77 Gel 8 Picks 78-88 Gel 9 Picks 89-96

  12. Origin PCR Origin PCRRedos

  13. Polyacrylamide Chaperone PCR Gel 2 Gel 1 M,M [Plate 6] A3-A4, A6-A9, B1, B3 M,M [Plate 6] B4-B6, B8-B11, C1

  14. Polyacrylamide Chaperone PCR Gel 4 Gel 3 M,M [Plate 6] C2-C12, D1 M,M [Plate 6] D2-D12

  15. Polyacrylamide Chaperone PCR Gel 5 Gel 6 M,M, [Plate 6] E2-E12, F2 M,M [Plate 6] F3-F6, F8-F11

  16. Polyacrylamide Chaperone PCR Gel 7 Gel 8 M,M [Plate 6] G2-G11, H2, H6 M,M [Plate 6] H7-H10 [Plate 5] A3, A10, B1-B2

  17. Polyacrylamide Chaperone PCR Gel 9 Gel 10 M,M, [Plate 5] B4-B5, B7-B11, C1 M,M, [Plate 5] C5, C8-C11, D1-D3

  18. Polyacrylamide Chaperone PCR Gel 12 Gel 11 M,M [Plate 5] D6, A8, D7-D9, D11-D12, E1, E3-E4, E6-E7M,M [Plate 5] E9, E11, F4, F6-F8, F10, F12, G3, G7-G8, G10

  19. Polyacrylamide Chaperone PCR Gel 14 Gel 13 M,M [Plate 5] H1, H4-H5, H7, H9-H10 [Plate 4] A1-A2, A5-A8 M,M [Plate 4] A11-A12, B1-B2, B4, B6-B8

  20. Polyacrylamide Chaperone PCR Gel 15 Gel 16 M,M [Plate 4] B11-B12, C4-C5, C8-C10, C12, D1-D2, D4-D5 M,M [Plate 4] D6-D12, E7

  21. Polyacrylamide Chaperone PCR Gel 17 Gel 18 M,M [Plate 4] E8-E12, F1-F3 M,M [Plate 4] F6-F12, G2, G4, G6-G8

  22. Polyacrylamide Chaperone PCR Gel 19 Gel 20 M,M, [Plate 4] G9-G12, H1, H3, H5-H10 M,M, [Plate 4] H12

  23. Agarose Repeat Chaperone PCR Gel 1 Gel 2 Gel 3

  24. Agarose Repeat Chaperone PCR Gel 4 Gel 5 Gel 6

  25. Agarose Repeat Chaperone PCR Gel 7 Gel 8 Gel 9

  26. Agarose Repeat Chaperone PCR Gel 10

  27. Plate 4 Plate 5 Plate 6

  28. Plate 4 Plate 5 Plate 6

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