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Genetics and Development

Genetics and Development. Embryology & Genetics. How did the work of Boveri and Stevens support the chromosomal hypothesis of inheritance?. Embryology & Genetics. In what way were Gluecksohn-Shoenheimer and Waddington early pioneers in developmental genetics?. Genomic Equivalence.

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Genetics and Development

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  1. Genetics and Development

  2. Embryology & Genetics • How did the work of Boveri and Stevens support the chromosomal hypothesis of inheritance?

  3. Embryology & Genetics • In what way were Gluecksohn-Shoenheimer and Waddington early pioneers in developmental genetics?

  4. Genomic Equivalence • What is meant by a totipotent nucleus?

  5. Genomic Equivalence • How were Briggs and King able to show that a blastula nucleus was totipotent? • What if more advanced nuclei were used?

  6. Genomic Equivalence

  7. Genomic Equivalence • How does the potency of somatic cells from Rana pipens compare with those from Xenopus laevis? • How did Gurdon’s transplant technique differ from Briggs and King?

  8. Genomic Equivalence • How was the first mammal cloned?

  9. Genomic Equivalence

  10. Differential Gene Expression • How do we know that only a small part of the genome is expresse in each cell?

  11. Molecular Biology Techniques • How can northern blotting be used to obtain information about development?

  12. Molecular Biology Techniques

  13. Molecular Biology Techniques • What’s involved in reverse transcriptase-polymerase chain reaction (RT-PCR)?

  14. Molecular Biology Techniques • Why is RT-PCR valuable in developmental studies?

  15. Molecular Biology Techniques • For what are DNA microarrays used?

  16. Molecular Biology Techniques • What is involved in in situ hybridization?

  17. Molecular Biology Techniques Whole-mount in situ hybridization

  18. Molecular Biology Techniques • Transgenic cells & organisms • What techniques are used to insert DNA into cells? • Microinjection • Transfection • Electroporation • Retroviral vector • P element

  19. Molecular Biology Techniques Producing chimeric mice

  20. Molecular Biology Techniques • What’s the difference between transgenic and “knockout” mice?

  21. Molecular Biology Techniques • What is antisense mRNA and how may it be used?

  22. Molecular Biology Techniques • What is RNA interference and how is it used?

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