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Application of TT-full-COLD-PCR for Tm-Increasing Mutation Analysis in TP53 Exon 8

This supplementary figure illustrates the application of TT-full-COLD-PCR to analyze Tm-increasing mutations in the TP53 gene, specifically a series of T>G mutations at 4% and 2% frequency. The efficacy of conventional PCR versus TT-full-COLD-PCR is compared, demonstrating the improved amplification and sensitivity of TT-full-COLD-PCR for detecting low-frequency mutations. This method holds promise for enhancing mutation detection in clinical samples where conventional methods may fail.

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Application of TT-full-COLD-PCR for Tm-Increasing Mutation Analysis in TP53 Exon 8

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  1. Conventional PCR TT-full-COLD PCR wt 4% T>G 2% T>G ~27% T>G ~27% T>G PFSK-1 Supplementary Figure 2. Application of TT-full-COLD-PCR for Tm-increasing mutation. A 4% and 2% T>G mutation, TP53 exon 8, amplified by conventional PCR or TT-full-COLD-PCR.

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