Gene-Specific Overgos for Cotton BIBAC Library Screening

Feb 27, 2025

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Explore the use of gene-specific overgos in screening the Upland cotton cv. TM-1 BIBAC library for targeted gene discovery and characterization.

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Gene-Specific Overgos for Cotton BIBAC Library Screening

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Table S1Gene-specificovergos used for the Upland cotton cv. TM-1 BIBAC library screening

Supplementary Table S1. List of oligonucleotide primers used for amplification

Supplementary Table S1. List of oligonucleotide primers used for amplification of RSV RNA1 and RNA2 segments. Supplementary Table S2. GenBank accession numbers of RSV RNA 1 and RNA2 segments used in the study. Jonson et al. Supplementary Fig. S1. (A) RNA2 ORF1. (B) RNA2 ORF2. BA2-JB.

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Table S1

Table S1. Table S1. The primer list used in the present study.

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Supplementary Table I . Gene-specific primers used for quantitatative real-time PCR

Supplementary Table I . Gene-specific primers used for quantitatative real-time PCR.

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Table S1. Primers used for semiquantitative RT-PCR

Table S1. Primers used for semiquantitative RT-PCR. 4. 4. 10. 10. 3. 3. 10. 10. 93.4. 96.6. 2. 2. 10. 10. 1. 1. 10. 10. 0. 0. 10. 10. 0. 1. 2. 3. 4. 0. 1. 2. 3. 4. 10. 10. 10. 10. 10. 10. 10. 10. 10. 10.

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(Table S1)

(Table S1). B. A. B. E. F. anterior. C. E. posterior. F. (Figure S1). ON. lCa. lCa. m. lo. SOG. PB. C. D. E. D. lCa. CC. Pe. CC. 1.E+02. 1.E+01. 1.E+00. 1.E-01. Relative Kakugo content. 1.E-02. 1.E-03. 1.E-04. days. 0. 1. 4. 6. 9. 14. (4). (3). (3).

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Supplementary Table S1

Relationship between the miRNA-21 in plasma and primary ESCC tissues. Supplementary Table S1. Relationship between the miRNA-375 in plasma and primary ESCC tissues. Supplementary Table S2. 1.0. 1.0. 0.8. 0.8. 0.6. 0.6. 0.4. 0.4. 0.2. 0.2. miR-21 AUC=0.618. miR-375 AUC=0.807. 0. 0.

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Supplemental Table S1

Supplemental Table S1. Table S1. Quantitative real-time RT-PCR primer sequences for genes used in the publication. All sequences are listed in the 5’ – 3’ direction. Supplemental Fig. S1. Induced by E2 and IGF-I. 3hr. 24hr. Repressed by E2 and IGF-I. 3hr. 24hr.

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Table S1. Organisms used for lytA specificity studies

Table S1. Organisms used for lytA specificity studies. lytA 173bp PCR product. Organism. Strain. Streptococcus pneumoniae. ATCC 49619. positive. Streptococcus pneumoniae. ATCC 6303. positive. Streptococcus pneumoniae. positive. ATCC 6305. Streptococcus pneumoniae. positive.

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Table S1. Primer and probes sets used for mcrA gene surveys

Table S1. Primer and probes sets used for mcrA gene surveys. Table S2. Oligonucleotide probes sequence targeting the Methyl Coenzyme M reductase subunit A gene ( mcrA ).

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TABLE S1

TABLE S1.

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Table. S1

Table. S1.

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Table S1

0.023. 0.023. 0.23. 0.23. 2.3. 2.3. 23 c. 23. 5.5. 24.9. 65.07. 4.7. 44.9. 171.3. 1.2. 1.5. 2.3. 1.1. 1.1. 2.1. Table S1. 5-Me-Tetrahydrofolate (5-MeTHF). Folic acid (FA). Tetrahydrofolate (THF). IC 50 s (nM) in physiological folate (FA 0.023 µM).

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Supplementary Table S1.

Supplementary Table S1. Primer sequences used for PCR amplification of DDAH1, DDAH2 and tubulin message. Supplementary Figures. s1A. s1B. s1C. s1D.

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Table S1. Functional gene grouping.

Table S1. Functional gene grouping. APOPTOSIS : ABL, BRCA1, CIDIA, GADD45 α , GADD45 γ , GML, IP6K3, PCBP4, AIFM1 (PDCD8), PPP1R15A, RAD21, p53, p73. CELL CYCLE ARREST : CHK1, CHK2, DDIT3 (CHOP), GADD45 α , GML, GTSE1, HUS1, MAP2K6, MAPK12, PCBP4, PPP1R15A, RAD17, RAD9A, SESN1, ZAK.

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Table S1

Table S1.

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Supplementary Table S1 . Summary of cell lines used.

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Table S1: Parameters used for collection of the switchgrass internodes.

Table S1: Parameters used for collection of the switchgrass internodes. Values are means ± SE (n=30).

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Supplemental Table S1

Supplemental Table S1. Complete list of LC-MS myc interating protiens in the MCF7 cells, proteins highlighted in red are those differentially expressed from the LCC1 and LCC1 EGF treated cells. Supplemental Table S2.

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SUPPLEMENTARY Table S1. Gene list for gene clusters 2 and 5

SUPPLEMENTARY Table S1. Gene list for gene clusters 2 and 5. SUPPLEMENTARY Table S2. HDGF and TIA-1 expression levels in 293 cell lines.

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Supplemental Table S1

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