Gene Annotation and Confusion Resolution Study
Explore gene annotation challenges faced during genomic analysis and solutions to clarify coding potential. Discover overlaps, deletions, and matches in DNA sequencing data.
Gene Annotation and Confusion Resolution Study
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Presentation Transcript
Annotation Update By: Sandra, Tal, Morella
MarQuardt: A3 Phage Annotated • Genes 1-20 • Tal: Genes 1-8 • Morella: Genes 9-16 • Sandra: Genes 17-20
Areas of Confusion • Is this gene’s coding potential all included?
More Areas of Confusion • It was hard to see which gene was which between DNA Master and the Phamerator Maps • Gene 1 was confusing as to whether it was actually a gene or not because of all the factors that played in • Gene 1 was also difficult because it was the first gene annotated
Genes Added or Deleted • No genes were added to our annotated genes • Gene 1 was deleted -Lack of start codon -No Blast match -Gene is too short -Coding potential is just a peak
Interesting Features • There are 3 tRNAs in our section to annotate • We have not annotated them yet
Interesting features • Gene 5 and 7 had an overlap with the previous gene, so they were both changed and their SD scores and ORFs were lowered Gene 5 Gene 7
More Interesting Features • For the blast match, genes 10-16 match with Vix or Bxz2 • Only gene 9 matches with JHC117 in our sequence annotated • For the blast match, genes 17-20 all matched with Bxz2
Gene 8 Pham Circle • This map displays that Pham 4284 is found in many different Clusters
Questions Remaining • Coding potential of gene 16 • Genes 17-20 were straightforward and only a few start codons were changed • The 3 tRNAs need to be annotated
