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Lab #10: Exercise and CO 2 production. Introduction:. Cellular respiration is the process that takes place your cells; it begins in the cytoplasm and ends in the mitochondria. Your cells go through cellular respiration because they need the energy that is stored in glucose.
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Introduction: • Cellular respiration is the process that takes place your cells; it begins in the cytoplasm and ends in the mitochondria. • Your cells go through cellular respiration because they need the energy that is stored in glucose. • The energy of one glucose molecule is converted into 38 ATPs.
The harder your cells work the more ATP they need. • So, they will break down more glucose, use more oxygen and in turn produce more carbon dioxide. • An acid-base indicator such as bromthymol blue can be used to indicate the presence of carbon dioxide. • Bromthymol blue changes to yellow or green when carbon dioxide is present
Problem: How will exercise affect your body’s production of carbon dioxide? • Hypothesis: If I exercise then, ________. • Materials: • Straws • Bromthymol blue • Test tube • Stop watch
Procedure: • Put 5 ml of bromthymol blue solution into the graduated cylinder and pour into a test tube. • Your partner will time you during this step. When your partner says “go”, slowly blow air through a straw into the bromthymol blue solution. Caution: Do not inhale through the straw. • When the solution changes color, your partner should say “stop” and then record how long the color change took.
Jog in place for 2 minutes. Have your partner rinse out the graduated cylinder and add 5mL of fresh bromthymol blue to the graduated cylinder. • After you have completed 2 minute of jogging in place, slowly blow air into the bromthymol blue. Have your partner time the color change and record your time. • Trade roles with your partner. Repeat steps 1 through 5.
Data: • Time it takes to change solution from blue to yellow.
Conclusion: • Summarize the lab activity. Retell the procedure in your own words. • How did exercise affect the time for the solution to change color? • Did the results support your hypothesis? • What process in your body produces carbon dioxide? • How does exercise affect carbon dioxide production?
What is the function of the mitochondria? • What is the equation for cellular respiration? • Why did we use bromthymol blue in this experiment? • What are the products of cellular respiration? • How many molecules of ATP, would 3 molecules of glucose produce? Show your work!