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Genetic Engineering Techniques: Molecular Cloning Strategies and Applications

Explore various restriction enzymes like SpeI, NotI, NheI, and their uses in gene manipulation. Learn about pGEM vectors, gene expression, and plasmid isolation methods in molecular biology research.

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Genetic Engineering Techniques: Molecular Cloning Strategies and Applications

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  1. SpeI NotI NotI NheI SpeI pGEM-PlacZ ilvIH SpeI NheI+SpeI pGEM-ilvIH NheI SpeI ilvC SpeI NheI+SpeI pGEM-ilvC NheI SpeI ilvD SpeI NheI+SpeI pGEM-ilvD NheI SpeI kivd SpeI NheI+SpeI pGEM-kivd NheI SpeI adh SpeI NheI+SpeI pGEM-adh SpeI (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) NotI NotI IlvIH IlvC IlvD kivd adh pGEM-PlacZ-ilvIH-ilvC-ilvD-kivd-adh NotI+Klenow ScaI pBR322 SpeI (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (SpeI/NheI) (ScaI/NotI) (ScaI/NotI) IlvIH IlvC IlvD kivd adh pBR-iBO Supplementary Fig. 1.

  2. A PstI PstI PstI 4.2 kb adc ldhA PstI PstI ldhAadc aac(3)IV 6.0 kb B Apr adc [Probe] ldhAadc ldhA adc ldhA ldhA 6.0 kb 4.2 kb Supplementary Fig. 2.

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