Dual Taxon Annotation

1. Dual Taxon Annotation Amelia Ireland and Jane Lomax

2. Dual Taxon Annotation • Annotation guide states Taxon: taxonomic identifier(s) • For cardinality 1, the ID of the species encoding the gene product. • For cardinality 2, the first ID is that of the species encoding the gene product; the second ID is that of the species using the gene product. • Currently unused

3. Dual Taxon Annotation • Suggest redefining as • For cardinality 2, the first ID is that of the species encoding the gene product; the second ID is that of the species in which the gene product is acting. • Can be used for component or process terms

4. Dual Taxon Annotation • Bacteria living as endosymbiont in plant cell; secretes protein esp1 into host cytoplasm • Bacteria taxon 666 • Host taxon 123 • Annotation of esp1: esp1 host cell cytoplasm taxon:666|taxon:123

5. Dual Taxon Annotation • Bacteria secretes protein bad1 which kills the host cell • Annotation: bad1 killing of host cells taxon:666|taxon:123

6. Dual Taxon Annotation • Bacterial protein lig1 interacts with rec5 from bacteria of taxon 999, enabling them to form a biofilm • Annotation: lig1 multi-species biofilm formation taxon:666|taxon:999 rec1 multi-species biofilm formation taxon:999|taxon:666

7. Dual Taxon Annotation • Bacterial protein reg1 interacts with host protein met3, regulating metabolic processes in the host reg1 regulation of metabolism in host taxon:666|taxon:123 met3 regulation of metabolism by other organism taxon:123|taxon:666

8. Dual Taxon Annotation • Implementation: • Ensure GO DB can store second taxon • Enable display in AmiGO • Amend annotation documentation • Alter annotation tools • Annotations correct without second taxon