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Captive Broodstock Program for Sockeye Salmon: Spawning Review and Genetic Diversity Goals

This document outlines the goals and methodologies of the Sockeye Salmon Captive Broodstock Program. Aiming to prevent population extinction, the program prioritizes conserving genetic diversity and increasing individual numbers. The process involves transitioning from a captive broodstock to an integrated broodstock system to eventually delist the population. Key techniques include equalizing family size, maximizing breeders, and minimizing inbreeding. Detailed protocols for spawning, including the collection of genetic samples and management of larvae through incubation, are defined to address challenges and improve stock revitalization.

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Captive Broodstock Program for Sockeye Salmon: Spawning Review and Genetic Diversity Goals

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  1. Sockeye Salmon Captive Broodstock Program:Spawning Review

  2. Program goals Avoid population extinction using captive technology. Tier 1 Conserve population genetic diversity. Increase the number of individuals in the population. Move from a captive broodstock to an integrated broodstock and eventually de-list the population Tier 2

  3. Spawning Redfish Lake Sockeye Salmon • Captive broodstock spawning principles: • Equalize family size • Factorial Spawning • Maximize the number of breeders per generation • - Minimize inbreeding (relatedness)

  4. Pre-season….. Genetic samples are processed for the Age-3 captive broodstock.

  5. Pre-season….. Ultrasound all Age-3 captive broodstock to determine maturation status and sex.

  6. Pre-season….. Genotype returning anadromous sockeye to determine which fish to incorporate into the captive broodstock.

  7. Pre-season….. Develop the spawning matrix.

  8. Spawning Redfish Lake Sockeye Salmon Preparation: • Determine which males are running. • Determine which females are ready. • Develop crosses.

  9. Spawning Redfish Lake Sockeye Salmon Female Station: • Strip green eggs into one gallon zip lock • Add oxygen • Place eggs in cooler

  10. Spawning Redfish Lake Sockeye Salmon Male Station: • Identify males needed to cross with female • Collect milt in whirl-pak • Check motility of milt. • Add oxygen • Place in cooler

  11. Spawning Redfish Lake Sockeye Salmon Fertilization Station: • Split green eggs into three equal groups. • Fertilize eggs with milt from appropriate male. • 20 minute iodophor disinfection.

  12. Spawning Redfish Lake Sockeye Salmon Incubation: • Label incubator. • Return to incubation area.

  13. Spawning Redfish Lake Sockeye Salmon The Next Generation: • Equalize Family Size • Select one or two eggs from each spawn cross.

  14. Spawning Redfish Lake Sockeye Salmon Program Challenges: • Spawn Timing – Anadromous vs Captive. • Isolation between Anadromous and Captive stocks. • Low volumes of milt from captive males. • Delay before fertilization occurs. • Small number of sockeye spawned per day. Questions

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