10 likes | 120 Vues
This study investigates the enhanced tolerance to nickel (Ni) conferred by the disruption of Tna1 and Fur4 transporters in various yeast strains, including wild type (WT), tna1∆, and fur4∆. Growth assessments were performed using spot assays on metal-free YPD medium and on YPD supplemented with nickel chloride (NiCl2) at a concentration of 3.5 mM. The results reveal significant differences in growth responses, highlighting the roles of Tna1 and Fur4 in metal tolerance.
E N D
Additional data file 6 - Enhanced Ni tolerance conferred by disruption of the Tna1 and Fur4 transporters Growth of WT, tna1∆ and fur4∆ strains was examined by spot assays carried out on metal-free YPD and on the same medium supplemented with NiCl2 (3.5 mM).