NZ9000(pNZ8020)

1. dnaK groEL recA 1:1 1:10 1:50 RNA DNA 1:1 1:10 1:50 RNA DNA 1:1 1:10 1:50 RNA DNA NZ9000(pNZ8020) NZ9000(pNZotsBA) NZ9000(pNZtpo) clpP tuf 1:1 1:10 1:50 RNA DNA 1:1 1:10 1:50 RNA DNA NZ9000(pNZ8020) NZ9000(pNZotsBA) NZ9000(pNZtpo) Supplementary Material Figure S1.

2. Figure S1. Transcriptional studies of stress response genes in L. lactis. Strains NZ9000(pNZ8020), NZ9000(pNZotsBA), and NZ9000(pNZtpo) were grown in CDM with 1% glucose (w/v) and without pH control (initial pH 6.5). PCR experiments were performed with chromosomal DNA from each strain (DNA, positive control), RNA treated without reverse transcriptase (RNA, negative control) and cDNA resulting from the reverse transcription reaction (RT). The cDNA samples were diluted 1:10 and 1:50 and used as template in the PCR analysis. RT-PCR experiments were performed using primers designed to amplify intragenic regions of dnaK (425 bp), groEL (422 bp), recA (451 bp), and tufA (471 bp). The house-keeping gene, tufA, was used as a control in the reverse transcription-PCR experiments. The experiments were performed with two or three independent cultures.