Category 1

1. Category 1 10 20 30 40 50 Category 2 10 20 30 40 50 Category 3 10 20 30 40 50 Category 4 10 20 30 40 50 Category 5 10 20 30 40 50

2. Category 1 questions follow

3. Category 1 a. b. c. Match the following Linkage map Pedigree karyotype 10

4. Category 1 a. b. c. Pedigree Linkage map karyotype 10

5. Category 1 Match the following Linkage map Pedigree karyotype 20 Indicates the autosomes and sex chromosomes of an individual Shows the location of genes on a chromosome Indicates how traits are inherited in a family

6. Category 1 Linkage map -Shows the location of genes on a chromosome Pedigree - Indicates how traits are inherited in a family Karyotype - Indicates the autosomes and sex chromosomes of an individual 20

7. Category 1 30 To determine if an individual with a dominant phenotype is homozygous or heterozygous you would do a test cross with _________________

8. Category 1 30 To determine if an individual with a dominant phenotype is homozygous or heterozygous you would do a test cross with _________________ Homozygous recessive

9. Category 1 40 A ring of DNA found in a bacterial cell is called A restriction enzyme Plasmid PCR

10. Category 1 A ring of DNA found in a bacterial cell is called A restriction enzyme Plasmid PCR 40

11. Category 1 Which is NOT correct? Entire DNA strands move through the gel The fragments are separated based on charge/length Current is used to separate the fragments with the negative fragments moving away from wells 50 Read from the bottom to the top

12. Category 1 Which is NOT correct? Entire DNA strands move through the gel- only fragments move The fragments are separated based on charge/length Current is used to separate the fragments with the negative fragments moving away from wells All are correct 50 Read from the bottom to the top

13. Category 2 questions follow

14. Category 2 10 The entire collection of genes within human cells is called the ________________, while the map which indicates which cells are inherited in a specific family is a ______________.

15. Category 2 10 The entire collection of genes within human cells is called the _Human genome___, while the map which indicates which cells are inherited in a specific family is a __Pedigree__.

16. Who are the parents of the baby? Remember- start from the bottom 20

17. Category 2 Who are the parents of the baby? B 20

18. Category 2 30 What scientific term do you use for a goat that produces spider silk in its milk? Weird Cool Transgenic Recombinant DNA

19. Category 2 30 What scientific term do you use for a goat that produces spider silk in its milk? Weird Cool Transgenic Recombinant DNA

20. Category 2 40 When crossing similar organisms, what technique would probably produce bigger and larger offspring? Inbreeding hybridization

21. Category 2 40 When crossing similar organisms, what technique would probably produce bigger and larger offspring? Inbreeding Hybridization (Hybrid vigor)

22. Category 2 50 If you want to breed for extremes in a trait you would use Inbreeding hybridization

23. Category 2 50 If you want to breed for extremes in a trait you would use Inbreeding- (ex smaller horses) hybridization

24. Category 3 questions follow

25. Uses heat to make many copies of DNA • Makes identical copies of organisms • Breaks up and separates DNA fragments to produce DNA fingerprints (profiles) Category 3 Match PCR Electrophoresis Cloning 10

26. PCR_ Uses heat to make many copies of DNA • Cloning- Makes identical copies of organisms • Electrophoresis-Breaks up and separates DNA fragments to produce DNA fingerprints (profiles) Category 3 10

27. Category 3 20 How are PCR and electrophoresis similar?

28. Category 3 20 How are PCR and electrophoresis similar? They both may cut up DNA into fragments

29. Category 3 30 ______________ cut the DNA into fragments Restriction enzymes Recombinant DNA Plamids

30. Category 3 30 ______________ cut the DNA into fragments Restriction enzymes Recombinant DNA Plamids

31. Category 3 40 If a normal gene is added into cells with defective genes, it is called Cloning Gene therapy PCR electrophoresis

32. Category 3 40 If a normal gene is added into cells with defective genes, it is called Cloning Gene therapy PCR electrophoresis

33. Category 3 50 How is DNA fingerprinting used in crime analysis?

34. Category 3 50 How is DNA fingerprinting used in crime analysis? Law enforcement workers use unique DNA fingerprint patterns to determine whether suspects have been at a crime scene. DNA samples can be obtained from hair, blood, skin, or semen. Because no two individuals have the same DNA sequence, samples from the suspects can be matched with samples taken at the crime scene.

35. Category 4 questions follow

36. Category 4 10 Which of these is NOT an example of Genetic Engineering Adding protein DNA to rice Adding spider silk production DNA to goats Cross breeding to produce horses smaller than 2 feet tall Adding enzyme DNA to bacteria to breakdown oil

37. Category 4 10 Which of these is NOT an example of Genetic Engineering Adding protein DNA to rice Adding spider silk production DNA to goats Cross breeding to produce horses smaller than 2 feet tall Adding enzyme DNA to bacteria to breakdown oil

38. Category 4 20 List the procedural steps in order to produce a transgenic organism

39. Category 4 20 List the procedural steps in order to produce a transgenic organism Identify wanted gene Use restriction enzyme to remove wanted gene Add wanted gene to vector Transfer Recombinant DNA into bacterial cell

40. Category 4 30 Place in the correct blank. Gene therapy, genetic counselor, amniocentesis If a genetic disease runs in your family, you may see a ____________for advise. They may request the test _____________to determine if an unborn child has certain types of disease. If the child carries a genetic disease then _______________ may be used to treat it.

41. Category 4 30 If a genetic disease runs in your family, you may see a _genetic counselor, _for advise. They may request the test __amniocentesis _to determine if an unborn child has certain types of disease. If the child carries a genetic disease then __Gene therapy, _ may be used to treat it.

42. Category 4 40 The human Genome project required the use of PCR Gel electrophosesis Automated gene sequencers All of these

43. Category 4 40 The human Genome project required the use of PCR Gel electrophosesis Automated gene sequencers (remember Jurassic Park) All of these

44. Category 4 50 While the human genome may have about 35,000 – 40, 000 genes on its 46 chromosomes, only _______ % between human individuals is different.

45. Category 4 50 While the human genome may have about 35,000 – 40, 000 genes on its 46 chromosomes, only _______ % between human individuals is different. 0.1 % difference

46. Category 5 questions follow

47. Category 5 10 What is a biological vector?

48. Category 5 10 What is a biological vector? A virus or bacteria (plasmid) that carries foreign DNA into a host cell

49. Category 5 20 What is a mechanical vector?

50. Category 5 20 What is a mechanical vector? A micropippette or gene gun that carries foreign DNA into a host cell