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Five Complex Things about Legionella Analysis

Legionella is a kind of bacteria that causes severe breathing diseases, including pneumonia. One of the leading causes of a legionella outbreak could be contamination of conventional water pipelines. In such cases, it is observed that due to the lack of proper pipeline management and cleaning, the outbreak of such bacteria becomes evident. However, at such times, conducting a Legionella analysis becomes imperative.

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Five Complex Things about Legionella Analysis

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  1. Five Complex Things about Legionella Analysis Middle East Testing Services L.L.C

  2. Overview Legionella is a kind of bacteria that causes severe breathing diseases, including pneumonia. One of the leading causes of a legionella outbreak could be contamination of conventional water pipelines. In such cases, it is observed that due to the lack of proper pipeline management and cleaning, the outbreak of such bacteria becomes evident. However, at such times, conducting a Legionella analysis becomes imperative. There are different legionella analyses available out there. However, as effective as they are, they can also have a few difficult points about them. Read on to find more about it!

  3. In this method, the legionella bacteria derived from location samples are isolated on a media where its growth is supported. Though it is quite an effective legionella analysis method, there are a few difficult factors about it. Culture It requires a particular media for the bacteria to grow, such as BCYE Agar. However, not all laboratories may have it readily available. It is quite technically challenging. It is quite a slower process as it requires around five days to cultivate. It much depends on the personnel handling the treatment. 01 02 03 04

  4. Urinary antigen Primarily meant for seeking out the symptoms in a patient, this analysis depends on the detection of the bacteria within the victim’s urine. It is also one of the most common legionella analyses existing out there. However, even if it is one of the most common, it still has a few difficult facts about it: • It can only detect a particular serogroup of the bacteria (L. pneumophila). • When it comes to environmental isolates, it does not offer molecular comparisons.

  5. Serology Another methodology used for legionella analysis. The difficulties in these methodologies are as follows: • For this analysis, it requires a paired serum which needs to be collected in between the acute onset and two weeks, since the visibility of symptoms. Another set needs to be received after 3-6 weeks. • Because about 5 to 10% of the population has a titer of 1:≥256, it becomes hard to detect the bacteria. This is so because it becomes harder to distinguish between the bacteria and other pneumonia acquired through the community.

  6. PCR (positive polymerase chain reaction) Another well sought after analysis for legionella, this method is rapid and can be used to detect other serogroups of legionella other than Lp1. However, it does have one difficulty: • Different laboratories have different assays and can have limited commercial availability.

  7. DFA (Direct Fluorescent Antibody) Lastly, this method has become more specific in recent times. The difficulties one might face through this method are: • The regents needed for this analysis are a bit difficult to obtain. • It is more difficult technically, as many labs might not have the proper equipment for it. If you suspect a legionella outbreak in your area, then for the best legionella analysis in Dubai, get in touch with Metslab for the best services. Source:https://metslab.wordpress.com/2020/08/04/five-difficult-things-about-legionella-analysis/

  8. CONTACT US Middle East Testing Services L.L.C Address: Exhibition-Showroom No.4, Jurf Industrial Zone 1 Ajman UAE Phone: +971 589505070 / +971 589505077 Website:https://metslab.com/

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