1 / 11

Kathryn Mayer Arizona State University Dept. of Chemistry & Biochemistry

Effect of Autotrophic Metabolism on Dissolved Organic Carbon in Yellowstone National Park. Kathryn Mayer Arizona State University Dept. of Chemistry & Biochemistry. Extreme environment. Geochemical variability. Biodiversity. Why Carbon in Hot Springs?. Microbial metabolism

min
Télécharger la présentation

Kathryn Mayer Arizona State University Dept. of Chemistry & Biochemistry

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Effect of Autotrophic Metabolism on Dissolved Organic Carbon in Yellowstone National Park Kathryn Mayer Arizona State University Dept. of Chemistry & Biochemistry

  2. Extreme environment Geochemical variability Biodiversity Why Carbon in Hot Springs? • Microbial metabolism • Organic Carbon trends difficult to characterize

  3. “CH2O” + O2 CO2 + H2O Metabolism effects the organic carbon of a system • Autotrophy – primary producers, makes organic C • Photosynthetic • Chemosynthetic • Heterotrophy –uses C from autotrophs

  4. Hot Spring Chemosynthetic Zone Photosynthetic Zone ~73ºC HS: Filtered Hot Spring Water + Zone WaterDI: Deionized Water + Zone WaterUC: Filtered Hot Spring Water Project Heterotroph ‘06 Goal: Assess metabolism in 3 different hot spring/outflow systems by measuring amount of dissolved organic carbon (DOC)

  5. Hot Spring Chemosynthetic Zone Photosynthetic Zone ~73ºC

  6. Conceptual Model Photosynthesis Chemosynthesis UC Control Heterotrophy DI Control

  7. 680ºC “CH2O” CO2 NDIR Detector Methods • Use TOC-V Analyzer to measure amount of DOC

  8. Results… Not what we expected!

  9. We should have called this Project Autotroph NOT Heterotroph • Increases in DOC concentrations • Autotrophy • C production in Dark and Light bottles • Photosynthesis & Chemosynthesis • Genomic studies tell us there are autotrophs & heterotrophs present. This experiment does not show heterotrophy.

  10. Future Work • Study organic compound compositional changes with Electrospray Ionization Mass Spectrometry (ESI-MS) • Experiment being planned for Summer ‘08 Thank you… Dr. Hilairy Hartnett & Katie Alexander Women & Philanthropy

More Related