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Co-Transfection of Myc-Tagged β1 and Hsp90 Mutants: Immunoprecipitation and Western Blot Analysis

This study examines the interactions between myc-tagged full-length β1 and various deletion mutants of Hsp90 in COS cells, utilizing immunoprecipitation (IP) with a FLAG antibody. Subsequent Western blot (WB) analysis detected β1 proteins, evaluating their presence and confirming co-expression. Blots are shown from experiments repeated multiple times, ensuring consistency of results. The positions in the amino acid sequence are annotated, and contained sequences are highlighted. This data provides insights into the functionality and interaction of β1 with Hsp90 mutants.

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Co-Transfection of Myc-Tagged β1 and Hsp90 Mutants: Immunoprecipitation and Western Blot Analysis

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  1. myc-b1 FLAG hsp90 [530-724] FLAG hsp90 [1-309] FLAG hsp90 [1-456] FLAG hsp90 [1-530] IP: anti-FLAG WB: anti-b1 b1 b1 100 55.9 IP: anti-FLAG WB: anti-FLAG 38.3 kDa Cell lysate WB: anti-b1 100 55.9 Cell lysate WB: anti-FLAG 38.3 kDa Fig. S1. COS cells were co-transfected with the cDNAs encoding myc-tagged full-length β1 and various deletion mutants of hsp90. Immuno- precipitation (IP) was performed using a FLAG antibody and precipitates were tested for the presence of β1. Blots are representative of experiments repeated at least twice with identical results. Numbers identify the relative positions in the amino acid sequence; retained sequences are bracketed. MOLPHARM/2005/012682Fig S1

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