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Gene expression systems

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Gene expression systems. Why do we need/want to control gene expression?. Recombinant gene expression systems. Direct promoter fusions GAL4/UAS system GeneSwitch system Tet regulated expression. GAL4/UAS System. Binary Expression System (GAL4/UAS).

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Gene expression systems

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Presentation Transcript

  1. Gene expression systems Why do we need/want to control gene expression?

  2. Recombinant gene expression systems • Direct promoter fusions • GAL4/UAS system • GeneSwitch system • Tet regulated expression

  3. GAL4/UAS System

  4. Binary Expression System (GAL4/UAS)

  5. Fly lines available from the Bloomington Drosophila Stock Center For GAL4 and UAS manipulations: http://flystocks.bio.indiana.edu/

  6. Gene and Enhancer Trap Database for Zebrafish http://kawakami.lab.nig.ac.jp/ztrap/

  7. Gene and Enhancer Trap Database for Zebrafish http://kawakami.lab.nig.ac.jp/ztrap/

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