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Virology interests for the Maule Lab

Virology interests for the Maule Lab. Factors regulating plant cell-to-cell communication Host transcriptional and biochemical responses to virus infection Recessive resistance to potyvirus infection -------. Pea seed-borne mosaic virus (PSbMV; Potyvirus). Natural resistance genes.

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Virology interests for the Maule Lab

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  1. Virology interests for the Maule Lab • Factors regulating plant cell-to-cell communication • Host transcriptional and biochemical responses to • virus infection • Recessive resistance to potyvirus infection -------

  2. Pea seed-borne mosaic virus (PSbMV; Potyvirus)

  3. Natural resistance genes • Early ‘70s - natural resistance discovered in • Ethiopian pea accessions • Recessive resistances to potyviruses clustered • at multiple loci • Linkage group VI: • - PSbMV - P1 (sbm-1) • - PSbMV - P4 (sbm-4) • - PSbMV - L/P2 (sbm-3) • - Clover yellow vein virus (cyv-2) • - White lupin mosaic virus (wlv) • Linkage group II: • - PSbMV L1 (sbm-2) • - Bean yellow mosaic virus (mo) • - Bean common mosaic virus (bcm) • - Pea mosaic virus (pmv)

  4. Summary of research findings • sbm1 and sbm11 are mutant alleles of eIF4E. • The mutations lie in and around the m7G-cap –binding • pocket and interfere with cap-binding and protein • translation in yeast. • eIF4E as a susceptibility factor in pea has the unusual • role of trafficking with the virus from cell to cell and could • assist with virus cell-to-cell movement

  5. Structure of VPg Plochocka et al (1996) PNAS 93:12150

  6. Opportunities for collaboration • Exchange of information prior to publication • Sharing of crystallographic experiences/expertise

  7. Strategies for delivery • Reagents for marker-assisted selection for crops • Analysis of the molecular interaction between • VPg and eIF4E - crystallisation of VPg and complex • Prediction of resistance alleles for any potyvirus • -host interaction, and identification using TILLING

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