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HSC RNAseq Initial Results

Quality control analysis of RNA-seq results from Ariel Paulson, focusing on read trimming, alignment using Bowtie and Tophat, and unique read alignments. Segmented at 35bp segments, allowing only UM reads with 2 mismatches each.

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HSC RNAseq Initial Results

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  1. HSC RNAseqInitial Results Ariel Paulson

  2. Data QA/QC \\dm3\solexa-analysis\Li_Linheng\ave\Li_Linheng-2011-07-25\BB027YABXX\fastqc\fastqc_plots.htm Col2_3GFP Mega5FU READS TRIMMED TO 70bp long

  3. QC FAIL NO MATCH TRUE NO MATCH MULTI ALIGN UNIQUE ALIGN UNIQUE AT 0MM TAGS % READS

  4. Alignment using Bowtie 0.12.7 + Tophat, 1.3.1, only allow for UM reads. Broken into 2 segments @ 35bp each, 2mm each

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